tibljТуберкулез и болезни легкихTuberculosis and Lung Diseases2075-12302542-1506Медицинские знания и технологии10.21292/2075-1230-2020-98-5-32-36tiblj-1423Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСтруктура популяции генетического семейства Beijing Mycobacterium tuberculosis на территории Западной СибириThe population structure of Beijing family of Mycobacterium tuberculosis in Western SiberiaВязоваяА. А.VyazovayaA. A.annavyazovaya@gmail.comПасечникО. А.PasechnikO. A.ГерасимоваА. А.GerasimovaA. A.МокроусовИ. В.MokrousovI. V.Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. ПастераРоссияPasteur St. Petersburg Research Institute of Epidemiology and MicrobiologyRussian FederationОмский государственный медицинский университетРоссияOmsk State Medical UniversityRussian Federation2020020620209853236Copyright © Вязовая А.А., Пасечник О.А., Герасимова А.А., Мокроусов И.В., 20202020Вязовая А.А., Пасечник О.А., Герасимова А.А., Мокроусов И.В.Vyazovaya A.A., Pasechnik O.A., Gerasimova A.A., Mokrousov I.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tibl-journal.com/jour/article/view/1423Цель исследования

Цель исследования: оценить структуру штаммов генотипа Beijing Mycobacterium tuberculosis в Омской области, Западной Сибири.

Материалы и методы

Материалы и методы: изучено 325 штаммов M. tuberculosis, выделенных от больных туберкулезом в Омской области. Определяли принадлежность штаммов к генотипу Beijing, кластерам B0/W148 и 94-32. Проведен анализ локуса NTF и региона RD181. Древние штаммы Beijing были сполиготипированы и MIRU-VNTR-типированы по 12 локусам, и полученные профили сравнивали с международной базой данных SITVIT_WEB.

Результаты

Результаты. Среди 204 (62,7%) штаммов M. tuberculosis генотипа Beijing 176 (86,3%) штаммов были современной сублинии кластеров 94-32 (60,7%) и В0/W148 (25,0%). Внутри кластера 94-32 обнаружено 13 (10,5%) штаммов группы CAO. Штаммы древней сублинии Beijing RD181[+] и RD181[-] выявлены в 3,4% (7/204) и 10,3% (21/204) случаев соответственно. Все штаммы Beijing RD181[+] имели сполигопрофиль SIT269. Большинство штаммов древней сублинии были представлены MIRU-типами ‒ MIT135 и МIT642.

Заключение

Заключение. В Омской области Западной Сибири в популяции M. tuberculosis генотипа Beijing преобладают штаммы современной сублинии, распространенность древних штаммов данного генотипа (13,7%). Штаммы современной сублинии кластеров В0/W148, 94-32 CAO и штаммы древней сублинии Beijing характеризуются высоким уровнем множественной лекарственной устойчивости.

The objective

The objective: to study the structure of Beijing genotype family of Mycobacterium tuberculosis in Omsk Region, Western Siberia.

Subjects and methods

Subjects and methods: 325 strains of M. tuberculosis were studied, all of them were isolated from tuberculosis patients in Omsk Region. Strains were tested to identify if they belonged to Beijing genotype, B0/W148 and 94-32. Locus NTF and region RD181 were assessed. Ancient Beijing strains were analyzed by spoligotyping and MIRU-VNTR-typed at 12 loci, and the obtained profiles were compared with the international database of SITVIT_WEB.

Results

Results. Among 204 (62.7%) strains of M. tuberculosis from Beijing family, 176 (86.3%) of strains belonged to the modern sublineage of 94-32 (60.7%) and B0/W148 (25.0%) clusters. Inside 94-32 cluster, it was found that 13 (10.5%) strains belonged to CAO group. Strains of the ancient Beijing sublineages of RD181[+] and RD181[-] were detected in 3.4% (7/204) and 10.3% (21/204) cases, respectively. All of Beijing RD181[+] strains had SIT269 spoligoprofile. The majority of strains of the ancient sublineage were represented by the MIRU types – MIT135 and MIT642.

Conclusion

Conclusion. In Omsk Region of Western Siberia, strains of the modern sublineage prevail in the population of M. tuberculosis of Beijing family, the prevalence of ancient strains of this family is (13.7%). The high level of multiple drug resistance is typical of strains of the modern sublineage of B0/W148, 94-32 CAO clusters and strains of the ancient Beijing sublineage.

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The authors declare that there are no conflicts of interest present.