tibljТуберкулез и болезни легкихTuberculosis and Lung Diseases2075-12302542-1506Медицинские знания и технологии10.58838/2075-1230-2023-101-4-57-65tiblj-1745Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИммуногистохимическая и ультраструктурная визуализация коронавируса SARS-CoV-2 в тканях легких, лимфатических узлов и почек умерших пациентовImmunohistochemical and Ultrastructural Imaging of the SARS-CoV-2 Coronavirus in Lung, Lymph Node, and Kidney Tissues of Deceased PatientsОдиловА. А.OdilovA. A.

Одилов Акмалжон Адхамжонович - аспирант кафедры патологической анатомии медицинского института.

117198, Москва, ул. Миклухо-Маклая, д. 6

Тел.: +7 (495) 433-27-94

Akmalzhon A. Odilov - Post Graduate Student of Pathological Anatomy Department, Medical Institute.

6, Miklukho-Maklaya St., Moscow, 117198

Phone: +7 (495) 433-27-94

a.odilov.tma@gmail.comВолковА. В.VolkovA. V.

Волков Алексей Вадимович - доктор медицинских наук, доцент кафедры патологической анатомии медицинского института.

117198, Москва, ул. Миклухо-Маклая, д. 6

Тел.: +7 (495) 433-27-94

Aleksey V. Volkov - Doctor of Medical Sciences, Associate Professor of Anatomical Pathology Department, Medical Institute.

6, Miklukho-Maklaya St., Moscow, 117198

Phone: +7 (495) 433-27-94

alex.volkoff@gmail.comАбдуллаевА. О.AbdullaevA. O.

Абдуллаев Адхамжон Одилович - кандидат медицинских наук, заведующий Центром биомедицинских технологий.

100109, Ташкент, ул. Фараби, д. 2

Тел.: +998 (78) 150-78-25

Adkhamzhon O. Abdullaev - Candidate of Medical Sciences, Head of Biomedical Technology Center.

2, Farabi St., Tashkent, 100109

Phone: +998 (78) 150-78-25

adham_abdullaev@mail.ruШадмановА. К.ShadmanovA. K.

Шадманов Алишер Каюмович - доктор медицинских наук, профессор, ректор.

100109, Ташкент, ул. Фараби, д. 2

Тел.: +998 (78) 150-78-25

Alisher K. Shadmanov - Doctor of Medical Sciences, Professor, Rector.

2, Farabi St., Tashkent, 100109

Phone: +998 (78) 150-78-25

info@tma.uzСудариковА. Б.SudarikovA. B.

Судариков Андрей Борисович - доктор биологических наук, заведующий лабораторией молекулярной гематологии.

125167, Москва, Новый Зыковский проезд, д. 4

Тел.: +7 (495) 612-65-11

Andrey B. Sudarikov - Doctor of Biological Sciences, Head of Molecular Hematology Laboratory.

4, Novy Zykovsky Drive, Moscow, 125167

Phone: +7 (495) 612-65-11

andrey@sudarikov.netБабиченкоИ. И.BabichenkoI. I.

Бабиченко Игорь Иванович - доктор медицинских наук, профессор, заведующий кафедрой патологической анатомии медицинского института.

117198, Москва, ул. Миклухо-Маклая, д. 6

Тел.: +7 (495) 433-27-94

Igor I. Babichenko - Doctor of Medical Sciences, Professor, Head of Anatomical Pathology Department, Medical Institute.

6, Miklukho-Maklaya St., Moscow, 117198

Phone: +7 (495) 433-27-94

babichenko@list.ruФГАОУ ВО «Российский университет дружбы народов»РоссияRUDN UniversityRussian FederationТашкентская медицинская академияУзбекистанTashkent Medical AcademyUzbekistanФГБУ «Национальный исследовательский центр гематологии» МЗ РФРоссияNational Medical Research Center for Hematology, Russian Ministry of HealthRussian Federation20232908202310145765Copyright © Одилов А.А., Волков А.В., Абдуллаев А.О., Шадманов А.К., Судариков А.Б., Бабиченко И.И., 20232023Одилов А.А., Волков А.В., Абдуллаев А.О., Шадманов А.К., Судариков А.Б., Бабиченко И.И.Odilov A.A., Volkov A.V., Abdullaev A.O., Shadmanov A.K., Sudarikov A.B., Babichenko I.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tibl-journal.com/jour/article/view/1745Цель исследования

Цель исследования: иммуногистохимическая и электронно-микроскопическая визуализация коронавируса SARS-CoV-2 в тканях легких, лимфатических узлов и почек пациентов, умерших от COVID-19.

Материалы и методы

Материалы и методы. Для иммуногистохимических исследований в качестве материала использованы срезы фиксированных в формалине и заключенных в парафин тканей легких, лимфатических узлов и почек пациентов, умерших от COVID-19. Количественная оценка уровня вирусной нагрузки SARS-CoV-2 проведена с использованием оригинального способа кПЦР РВ и рассчитана по формуле: число копий SARS-CoV-2/число копий ABL1 х 100, выражена отношением истинного числа копий кДНК SARS-CoV-2 на 100 копий кДНК гена ABL1. Для морфологических исследований взяты нативные образцы тканей легких, лимфатических узлов и почек.

Результаты

Результаты. При иммуногистохимическом и электронно-микроскопическом исследовании частицы коронавируса SARS-CoV-2 были обнаружены в цитоплазме эндотелиальных клеток аэрогематического барьера легких, сосудистого клубочка почек, в цитоплазме макрофагов лимфатического узла, а также в цитоплазме лимфоцитов в просвете капилляров легких.

The objective

The objective: immunohistochemical and electron microscopic imaging of the SARS-CoV-2 coronavirus in lung, lymph nodes and kidney tissues of patients who died of COVID-19.

Subjects and Methods

Subjects and Methods. For immunohistochemical tests, specimens of sections of formalin-fixed and paraffin-embedded tissues of the lungs, lymph nodes and kidneys of patients who died from COVID-19 were used. Quantitative assessment of the SARS-CoV-2 viral load level was carried by the original RT qPCR and calculated by the formula: SARS-CoV-2 copy number/ABL1 х 100 copies, expressed as the ratio of the true number of SARS-CoV-2 cDNA copies per 100 copies cDNA of the ABL1 gene. For morphological tests, samples of native lung, lymph node and kidney tissues were taken.

Results

Results. Immunohistochemical and electron microscopic tests revealed particles of the SARS-CoV-2 coronavirus in the cytoplasm of endothelial cells of air-blood barrier of the lungs, the vascular glomerulus of the kidneys, in the cytoplasm of macrophages of the lymph node, and also in cytoplasm of lymphocytes in the lumen of lung capillaries.

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The authors declare that there are no conflicts of interest present.