tibljТуберкулез и болезни легкихTuberculosis and Lung Diseases2075-12302542-1506Медицинские знания и технологии10.58838/2075-1230-2025-103-6-96-103tiblj-1938Research ArticleОБЗОРЫREVIEWSВозможности применения трегалозных зондов для выявления микобактерий туберкулезаPossibilities of Using Trehalose Probes for Detection of Mycobacterium tuberculosisАмбарцумянО. А.AmbartsumyanO. A.

Амбарцумян Оганес Альбертович - Младший научный сотрудник научной лаборатории микробиологии; научный сотрудник лаборатории неравновесных электронных процессов 

127473, Москва, ул. Достоевского, д. 4, к. 2Тел.+7 (495) 631-15-15

Oganes A. Ambartsumyan - Junior Researcher of Research Microbiology Laboratory, Researcher of Laboratory of Nonequilibrium Electronic Processes

Build. 2, 4 Dostoevskiy St., Moscow, 127473Phone: +7 (495) 631-15-15

ogan-mail@mail.ruЕлисеевП. И.EliseevP. I.

Елисеев Платон Иванович - К. м. н., заведующий научной лабораторией микробиологии

127473, Москва, ул. Достоевского, д. 4, к. 2Тел.+7 (495) 631-15-15

Platon I. Eliseev - Candidate of Medical Sciences, Head of Microbiology Research Laboratory

Build. 2, 4 Dostoevskiy St., Moscow, 127473Phone: +7 (495) 631-15-15

EliseevPI@nmrc.ruСкурединаО. А.SkuredinaO. A.

Скуредина Олеся Александровна - Лаборант-исследователь научной лаборатории микробиологии

127473, Москва, ул. Достоевского, д. 4, к. 2Тел.+7 (495) 631-15-15

Olesya A. Skuredina - Researcher of Microbiology Research Laboratory

Build. 2, 4 Dostoevskiy St., Moscow, 127473Phone: +7 (495) 631-15-15

inori1626@gmail.comГостеваЕ. Ю.GostevaE. Yu.

Гостева Екатерина Юрьевна - Младший научный сотрудник научной лаборатории микробиологии

127473, Москва, ул. Достоевского, д. 4, к. 2Тел.+7 (495) 631-15-15

Ekaterina Yu. Gosteva - Junior Researcher of Microbiology Research Laboratory

Build. 2, 4 Dostoevskiy St., Moscow, 127473Phone: +7 (495) 631-15-15

GostevaEY@nmrc.ruhttps://orcid.org/0000-0001-6596-9777СамойловаА. Г.SamoylovaA. G.

Самойлова Анастасия Геннадьевна - Д. м. н., заместитель директора по научной работе

127473, Москва, ул. Достоевского, д. 4, к. 2Тел.+7 (495) 631-15-15

Anastasiya G. Samoylova - Doctor of Medical Sciences, Deputy Director for Research

Build. 2, 4 Dostoevskiy St., Moscow, 127473Phone: +7 (495) 631-15-15

a.samoilova.nmrc@mail.ruhttps://orcid.org/0000-0002-0637-7955ВасильеваИ. А.VasilyevaI. A.

Васильева Ирина Анатольевна - Д. м. н, профессор, директор, заведующая кафедрой фтизиатрии127473, Москва, ул. Достоевского, д. 4, к. 2Тел.+7 (495) 631-15-15

 

Irina A. Vasilyeva - Doctor of Medical Sciences, Professor, Director, Head of Phthisiology Department, Clinical Medicine Institute, Pirogov Russian National Research Medical University, Russian Ministry of Health 

Build. 2, 4 Dostoevskiy St., Moscow, 127473Phone: +7 (495) 631-15-15

vasil39@list.ruФГБУ «Национальный медицинский исследовательский центр фтизиопульмонологии и инфекционных заболеваний» МЗ РФ; Институт физики твердого тела имени Ю.А. Осипьяна Российской академии наукРоссияNational Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health; Osipyan Institute of Solid State Physics, Russian Academy of SciencesRussian FederationФГБУ «Национальный медицинский исследовательский центр фтизиопульмонологии и инфекционных заболеваний» МЗ РФ; Институт физики твердого тела имени Ю.А. Осипьяна Российской академии наукРоссияNational Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of HealthRussian FederationФГБУ «Национальный медицинский исследовательский центр фтизиопульмонологии и инфекционных заболеваний» МЗ РФРоссияNational Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of HealthRussian Federation202510012026103696103Copyright © Амбарцумян О.А., Елисеев П.И., Скуредина О.А., Гостева Е.Ю., Самойлова А.Г., Васильева И.А., 20262026Амбарцумян О.А., Елисеев П.И., Скуредина О.А., Гостева Е.Ю., Самойлова А.Г., Васильева И.А.Ambartsumyan O.A., Eliseev P.I., Skuredina O.A., Gosteva E.Y., Samoylova A.G., Vasilyeva I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tibl-journal.com/jour/article/view/1938

Одним из перспективных направлений в диагностике туберкулеза являются трегалозные зонды, способные селективно проникать в Mycobacterium tuberculosis (МБT). При этом зонды генерируют флуоресцентный сигнал, позволяя проводить таким образцом детекцию. Для анализа современного состояния и перспектив применения трегалозных зондов, как нового подхода к экспресс-диагностике Mycobacterium tuberculosis, проведен обзор научной литературы. Рассмотрены основные типы зондов: флуорогенные, построенные по схеме «флуорофор-тушитель» и фотоактивируемые. Трегалозные зонды обеспечивают селективную детекцию МБT за счет специфического поглощения трегалозы, встраивания в клеточную стенку и последующей активации флуоресценции. Зонды позволяют обнаруживать МБT в образцах мокроты без сложной пробоподготовки и этапов отмывки. Методика позволяет дифференцировать жизнеспособные и нежизнеспособные клетки МБT, а также может применяться для тестирования их лекарственной чувствительности. 

Trehalose probes seem to be a promising area of tuberculosis diagnosis, these probes are capable of selectively penetrating Mycobacterium tuberculosis. These probes generate a fluorescent signal, enabling detection of mycobacteria. To analyze the current state of knowledge and prospects of trehalose probes as a new approach for rapid detection of Mycobacterium tuberculosis, a systematic review of scientific literature was conducted. The main types of probes include fluorogenic probes, "fluorophore-quencher" based probes, and photoactivatable probes. Trehalose probes enable selective detection of mycobacteria due to specific trehalose uptake and incorporation into the cell wall, followed by fluorescence activation. These probes allow for the detection of mycobacteria in sputum samples without complex sample preparation or washing. The method allows differentiation of viable and non-viable cells and can also be applied for drug susceptibility testing. 

трегалозные зондыфлуорогенные красителидетекцияMycobacterium tuberculosisэкспресс-диагностика туберкулезафлуоресценцияtrehalose probesfluorogenic stainsdetectionMycobacterium tuberculosisrapid diagnostics of tuberculosisfluorescenceReferencesВасильева И.А. Достижения и перспективы инновационных исследований в области фтизиатрии // Вестник Российской академии наук. – 2025. – № 1. – С. 63-74. https://doi.org/10.31857/S0869587325010063Vasilyeva I.A. Achievements and prospects of innovative research in the field of phthisiology. Herald of the Russian Academy of Sciences, 2025, no. 1, pp. 63-74. (In Russ.) https://doi.org/10.31857/S0869587325010063Вахрушева Д.В., Васильева И.А. К вопросу о стандартизации и качестве лабораторных исследований для диагностики и контроля химиотерапии туберкулеза // Туберкулез и болезни легких. – 2018. – Т. 96, № 9. – С. 57-62. https://doi.org/10.21292/2075-1230-2018-96-9-57-62Vakhrusheva D.V., Vasilyeva I.A. About the standardization and quality of laboratory tests aimed at diagnostics and monitoring of tuberculosis chemotherapy. Tuberculosis and Lung Diseases, 2018, vol. 96, no. 9, pp. 57-62. (In Russ.) https://doi.org/10.21292/2075-1230-2018-96-9-57-62Мартынов В.И., Пахомов А.А. Флуоресцентные производные BODIPY как репортеры молекулярных процессов в живой клетке // Успехи химии. – 2021. – Т. 90, № 10. – С. 1213-1262.Martynov V.I., Pakhomov A.A. BODIPY derivatives as fluorescent reporters of molecular activities in living cells. Russian Chemical Reviews, 2021, vol. 90, no. 10, pp. 1213-1262. (In Russ.)Babu Sait M.R., Koliwer-Brandl H., Stewart J.A., Swarts B., Jacobsen M., Ioerger T., Kalscheuer R. PPE51 mediates uptake of trehalose across the mycomembrane of Mycobacterium tuberculosis // Sci. Rep. – 2022. – Vol. 12, № 1. – Р. 2097. https://doi.org/10.1038/s41598-022-06109-7Babu Sait M.R., Koliwer-Brandl H., Stewart J.A., Swarts B., Jacobsen M., Ioerger T., Kalscheuer R. PPE51 mediates uptake of trehalose across the mycomembrane of Mycobacterium tuberculosis. Sci. Rep., 2022, vol. 12, no. 1, pp. 2097. https://doi.org/10.1038/s41598-022-06109-7Backus K.M., Boshoff H.I., Barry C.S., Boutureira O., Patel M.K., D'Hooge F., Lee S.S., Via L.E., Tahlan K., Barry C.E., et al. Uptake of Unnatural Trehalose Analogs as a Reporter for Mycobacterium tuberculosis // Nat. Chem. Biol. – 2011. – Vol. 7, № 4. – Р. 228-235. https://doi.org/10.1038/nchembio.539Backus K.M., Boshoff H.I., Barry C.S., Boutureira O., Patel M.K., D'Hooge F., Lee S.S., Via L.E., Tahlan K., Barry C.E. et al. Uptake of unnatural trehalose analogs as a reporter for Mycobacterium tuberculosis. Nat. Chem. Biol., 2011, vol. 7, no. 4, pp. 228-235. https://doi.org/10.1038/nchembio.539Banahene N., Gepford D.M., Biegas K.J., Swanson D.H., Hsu Y.P., Murphy B.A., Taylor Z.E., Lepori I., Siegrist M.S., Obregón-Henao A., et al. A Far-Red Molecular Rotor Fluorogenic Trehalose Probe for Live Mycobacteria Detection and Drug-Susceptibility Testing // Angew. Chem. Int. Ed. – 2023. – Vol. 62, № 2. – Р. 202213563. https://doi.org/10.1002/anie.202213563Banahene N., Gepford D.M., Biegas K.J., Swanson D.H., Hsu Y.P., Murphy B.A., Taylor Z.E., Lepori I., Siegrist M.S., Obregón-Henao A. et al. A far-red molecular rotor fluorogenic trehalose probe for live mycobacteria detection and drug-susceptibility testing. Angew. Chem. Int. Ed., 2023, vol. 62, no. 2, pp. 202213563. https://doi.org/10.1002/anie.202213563Brown T., Chavent M., Im W. Molecular Modeling and Simulation of the Mycobacterial Cell Envelope: From Individual Components to Cell Envelope Assemblies // J. Phys. Chem. B. – 2023. – Vol. 127, № 51. – Р. 10941-10949. https://doi.org/10.1021/acs.jpcb.3c06136Brown T., Chavent M., Im W. Molecular modeling and simulation of the mycobacterial cell envelope: from individual components to cell envelope assemblies. J. Phys. Chem. B., 2023, vol. 127, no. 51, pp. 10941-10949. https://doi.org/10.1021/acs.jpcb.3c06136Chen W.C., Chang C.C., Lin Y.E. Pulmonary Tuberculosis Diagnosis Using an Intelligent Microscopy Scanner and Image Recognition Model for Improved Acid-Fast Bacilli Detection in Smears // Microorganisms. – 2024. – Vol. 12, № 8. – Р. 1734. https://doi.org/10.3390/microorganisms12081734Chen W.C., Chang C.C., Lin Y.E. Pulmonary tuberculosis diagnosis using an intelligent microscopy scanner and image recognition model for improved acid-fast bacilli detection in smears. Microorganisms, 2024, vol. 12, no. 8, pp. 1734. https://doi.org/10.3390/microorganisms12081734Dinnes J., Deeks J., Kunst H., Gibson A., Cummins E., Waugh N., Drobniewski F., Lalvani A. A systematic review of rapid diagnostic tests for the detection of tuberculosis infection // Health Technol. Assess. – 2007. – Vol. 11, № 3. – Р. 1-196. https://doi.org/10.3310/hta11030Dinnes J., Deeks J., Kunst H., Gibson A., Cummins E., Waugh N., Drobniewski F., Lalvani A. A systematic review of rapid diagnostic tests for the detection of tuberculosis infection. Health Technol. Assess., 2007, vol. 11, no. 3, pp. 1-196. https://doi.org/10.3310/hta11030Dong B., He Z., Li Y., Xu X., Wang C., Zeng J. Improved Conventional and New Approaches in the Diagnosis of Tuberculosis // Front. Microbiol. – 2022. – № 13. – Р. 924410. https://doi.org/10.3389/fmicb.2022.924410Dong B., He Z., Li Y., Xu X., Wang C., Zeng J. Improved conventional and new approaches in the diagnosis of tuberculosis. Front. Microbiol., 2022, no. 13, pp. 924410. https://doi.org/10.3389/fmicb.2022.924410Eke I.E., Abramovitch R.B. Functions of nitroreductases in mycobacterial physiology and drug susceptibility // J. Bacteriol. – 2025. – № 207. – Р. e0032624. https://doi.org/10.1128/jb.00326-24Eke I.E., Abramovitch R.B. Functions of nitroreductases in mycobacterial physiology and drug susceptibility. J. Bacteriol., 2025, no. 207, pp. e0032624. https://doi.org/10.1128/jb.00326-24Geng P., Hong X., Li X., Ni D., Liu G. Optimization of nitrofuranyl calanolides for the fluorescent detection of Mycobacterium tuberculosis // Eur. J. Med. Chem. – 2022. – № 244. – Р. 114835. https://doi.org/10.1016/j.ejmech.2022.114835Geng P., Hong X., Li X., Ni D., Liu G. Optimization of nitrofuranyl calanolides for the fluorescent detection of Mycobacterium tuberculosis. Eur. J. Med. Chem., 2022, no. 244, pp. 114835. https://doi.org/10.1016/j.ejmech.2022.114835Hong X., Geng P., Tian N., Li X., Gao M., Nie L., Sun Z., Liu G. From Bench to Clinic: A Nitroreductase Rv3368c-Responsive Cyanine-Based Probe for the Specific Detection of Live Mycobacterium tuberculosis // Anal. Chem. – 2024. – Vol. 96, № 4. – Р. 1576-1586. https://doi.org/10.1021/acs.analchem.3c04293Hong X., Geng P., Tian N., Li X., Gao M., Nie L., Sun Z., Liu G. From bench to clinic: A nitroreductase Rv3368c-responsive cyanine-based probe for the specific detection of live Mycobacterium tuberculosis. Anal. Chem., 2024, vol. 96, no. 4, pp. 1576-1586. https://doi.org/10.1021/acs.analchem.3c04293Kalscheuer R., Koliwer-Brandl H. Genetics of Mycobacterial Trehalose Metabolism // Microbiol. Spectr. – 2014. – № 3. – Р. MGM2-0002-2013. https://doi.org/10.1128/microbiolspec.MGM2-0002-2013Kalscheuer R., Koliwer-Brandl H. Genetics of mycobacterial trehalose metabolism. Microbiol. Spectr., 2014, no. 3, pp. MGM2-0002-2013. https://doi.org/10.1128/microbiolspec.MGM2-0002-2013Kamariza M., Keyser S.G.L., Utz A., Knapp B.D., Ealand C., Ahn G., Cambier C.J., Chen T., Kana B., Huang K.C., Bertozzi C.R. Toward Point-of-Care Detection of Mycobacterium tuberculosis: A Brighter Solvatochromic Probe Detects Mycobacteria within Minutes // JACS Au. – 2021. – № 9. – Р. 1368-1379. https://doi.org/10.1021/jacsau.1c00173Kamariza M., Keyser S.G.L., Utz A., Knapp B.D., Ealand C., Ahn G., Cambier C.J., Chen T., Kana B., Huang K.C., Bertozzi C.R. Toward point-of-care detection of Mycobacterium tuberculosis: a brighter solvatochromic probe detects mycobacteria within minutes. JACS Au., 2021, no. 9, pp. 1368-1379. https://doi.org/10.1021/jacsau.1c00173Kamariza M., Shieh P., Bertozzi C.R. Imaging Mycobacterial Trehalose Glycolipids. In: Methods in Enzymology. 1st ed. Imperiali B., Ed. Academic Press: New York, NY, USA, 2018. рр. 355-369.Kamariza M., Shieh P., Bertozzi C.R. Imaging Mycobacterial Trehalose Glycolipids. In: Methods in Enzymology. 1st ed. Imperiali B., eds. Academic Press, New York, NY, USA, 2018, рр. 355-369.Kamariza M., Shieh P., Ealand C.S., Peters J.S., Chu B., Rodriguez-Rivera F.P., Babu Sait M.R., Treuren W.V., Martinson N., Kalscheuer R., et al. Rapid detection of Mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe // Sci. Transl. Med. – 2018. – Vol. 10, № 430. – Р. eaam6310. https://doi.org/10.1126/scitranslmed.aam6310Kamariza M., Shieh P., Ealand C.S., Peters J.S., Chu B., Rodriguez-Rivera F.P., Babu Sait M.R., Treuren W.V., Martinson N., Kalscheuer R. et al. Rapid detection of Mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe. Sci. Transl. Med., 2018, vol. 10, no. 430, pp. eaam6310. https://doi.org/10.1126/scitranslmed.aam6310Kobayashi H., Ogawa M., Alford R., Choyke P.L., Urano Y. New Strategies for Fluorescent Probe Design in Medical Diagnostic Imaging // Chem. Rev. – 2010. – Vol. 110, № 5. – Р. 2620-2640. https://doi.org/10.1021/cr900263jKobayashi H., Ogawa M., Alford R., Choyke P.L., Urano Y. New strategies for fluorescent probe design in medical diagnostic imaging. Chem. Rev., 2010, vol. 110, no. 5, pp. 2620-2640. https://doi.org/10.1021/cr900263jKumar G., Narayan R., Kapoor S. Chemical Tools for Illumination of Tuberculosis Biology, Virulence Mechanisms, and Diagnosis // J. Med. Chem. – 2020. – Vol. 63, № 24. – Р. 15308-15332. https://doi.org/10.1021/acs.jmedchem.0c01337Kumar G., Narayan R., Kapoor S. Chemical tools for illumination of tuberculosis biology, virulence mechanisms, and diagnosis. J. Med. Chem., 2020, vol. 63, no. 24, pp. 15308-15332. https://doi.org/10.1021/acs.jmedchem.0c01337Li Y.X., Xie D.T., Yang Y.X., Chen Z., Guo W.Y., Yang W.C. Development of Small-Molecule Fluorescent Probes Targeting Enzymes // Molecules. – 2022. – Vol. 27, № 14. – Р. 4501. https://doi.org/10.3390/molecules27144501Li Y.X., Xie D.T., Yang Y.X., Chen Z., Guo W.Y., Yang W.C. Development of small-molecule fluorescent probes targeting enzymes. Molecules, 2022, vol. 27, no. 14, pp. 4501. https://doi.org/10.3390/molecules27144501Liu G., Li X., Hong X., Geng P., Sun Z. Detecting Mycobacterium tuberculosis using a nitrofuranyl calanolide-trehalose probe based on nitroreductase Rv2466c // Chem. Commun. – 2021. – Vol. 97, № 57. – Р. 12688-12691. https://doi.org/10.1039/d1cc05187cLiu G., Li X., Hong X., Geng P., Sun Z. Detecting Mycobacterium tuberculosis using a nitrofuranyl calanolide-trehalose probe based on nitroreductase Rv2466c. Chem. Commun., 2021, vol. 97, no. 57, pp. 12688-12691. https://doi.org/10.1039/d1cc05187cLiyanage S.H., Raviranga N.G.H., Ryan J.G., Shell S.S., Ramström O., Kalscheuer R., Yan M. Azide-Masked Fluorescence Turn-On Probe for Imaging Mycobacteria // JACS Au. – 2023. – Vol. 3, № 4. – Р. 1017-1028. https://doi.org/10.1021/jacsau.2c00449Liyanage S.H., Raviranga N.G.H., Ryan J.G., Shell S.S., Ramström O., Kalscheuer R., Yan M. azide-masked fluorescence turn-on probe for imaging mycobacteria. JACS Au., 2023, vol. 3, no. 4, pp. 1017-1028. https://doi.org/10.1021/jacsau.2c00449Mu R., Kong C., Yu W., Wang H., Ma Y., Li X., Wu J., Somersan-Karakaya S., Li H., Sun Z., et al. A Nitrooxidoreductase Rv2466c-dependent Fluorescent Probe for Rapid Mycobacterium tuberculosis Diagnosis and Drug Susceptibility Testing // ACS Infect. Dis. – 2019. – № 5. – Р. 1210-1219. https://doi.org/10.1021/acsinfecdis.9b00006Mu R., Kong C., Yu W., Wang H., Ma Y., Li X., Wu J., Somersan-Karakaya S., Li H., Sun Z. et al. A nitrooxidoreductase Rv2466c-dependent fluorescent probe for rapid Mycobacterium tuberculosis diagnosis and drug susceptibility testing. ACS Infect. Dis., 2019, no. 5, pp. 1210-1219. https://doi.org/10.1021/acsinfecdis.9b00006Murugasu-Oei B., Tay A., Dick T. Upregulation of stress response genes and ABC transporters in anaerobic stationary-phase Mycobacterium smegmatis // Mol. Gen. Genet. – 1999. – Vol. 262, № 4-5. – Р. 677-682. https://doi.org/10.1007/s004380051130Murugasu-Oei B., Tay A., Dick T. Upregulation of stress response genes and ABC transporters in anaerobic stationary-phase Mycobacterium smegmatis. Mol. Gen. Genet., 1999, vol. 262, no. 4-5, pp. 677-682. https://doi.org/10.1007/s004380051130Negri A., Javidnia P., Mu R., Zhang X., Vendome J., Gold B., Roberts J., Barman D., Ioerger T., Sacchettini J.C., et al. Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis // ACS Infect. Dis. – 2018. – № 4. – Р. 771-787. https://doi.org/10.1021/acsinfecdis.7b00111Negri A., Javidnia P., Mu R., Zhang X., Vendome J., Gold B., Roberts J., Barman D., Ioerger T., Sacchettini J.C. et al. Identification of a mycothiol-dependent nitroreductase from Mycobacterium tuberculosis. ACS Infect. Dis., 2018, no. 4, pp. 771-787. https://doi.org/10.1021/acsinfecdis.7b00111Rodriguez-Rivera F.P., Zhou X., Theriot J.A., Bertozzi C.R. Visualization of Mycobacterial Membrane Dynamics in Live Cells // J. Am. Chem. Soc. – 2017. – Vol. 139, № 9 – Р. 3488-3491Rodriguez-Rivera F.P., Zhou X., Theriot J.A., Bertozzi C.R. Visualization of mycobacterial membrane dynamics in live cells. J. Am. Chem. Soc., 2017, vol. 139, no. 9, pp. 3488-3491Stavropoulou K., Papanastasiou I.P. Overview of Small Molecules as Fluorescent Probes of Mycobacterium tuberculosis // ACS Omega. – 2024. – № 9. – Р. 31220-31227. https://doi.org/10.1021/acsomega.4c01992Stavropoulou K., Papanastasiou I.P. Overview of small molecules as fluorescent probes of Mycobacterium tuberculosis. ACS Omega, 2024, no. 9, pp. 31220-31227. https://doi.org/10.1021/acsomega.4c01992Steingart K.R., Henry M., Ng V., Hopewell P., Ramsay A., Cunningham J., Urbanczik R., Perkins M., Aziz M., Pai M. Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review // Lancet Infect. Dis. – 2006. – № 6. – Р. 570-581. https://doi.org/10.1016/S1473-3099(06)70578-3Steingart K.R., Henry M., Ng V., Hopewell P., Ramsay A., Cunningham J., Urbanczik R., Perkins M., Aziz M., Pai M. Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. Lancet Infect. Dis., 2006, no. 6, pp. 570-581. https://doi.org/10.1016/S1473-3099(06)70578-3Swarts B.M., Holsclaw C.M., Jewett J.C., Alber M., Fox D.M., Siegrist M.S., Leary J.A., Kalscheuer R., Bertozzi C.R. Probing the mycobacterial trehalome with bioorthogonal chemistry // J. Am. Chem. Soc. –2012. – Vol. 134, № 9. – Р. 16123-16126.Swarts B.M., Holsclaw C.M., Jewett J.C., Alber M., Fox D.M., Siegrist M.S., Leary J.A., Kalscheuer R., Bertozzi C.R. Probing the mycobacterial trehalome with bioorthogonal chemistry. J. Am. Chem. Soc., 2012, vol. 134, no. 9, pp. 16123-16126.Verschoor J.A., Baird M.S., Grooten J. Toward understanding the functional diversity of cell wall mycolic acids of Mycobacterium tuberculosis // Prog. Lipid Res. – 2012. – Vol. 51, № 4. – Р. 325-339. https://doi.org/10.1016/j.plipres.2012.05.002Verschoor J.A., Baird M.S., Grooten J. Toward understanding the functional diversity of cell wall mycolic acids of Mycobacterium tuberculosis. Prog. Lipid Res., 2012, vol. 51, no. 4, pp. 325-339. https://doi.org/10.1016/j.plipres.2012.05.002Wells W.A., Boehme C.C., Cobelens F.G., Daniels C., Dowdy D., Gardiner E., Gheuens J., Kim P., Kimerling M., Kreiswirth B., et al. Alignment of new tuberculosis drug regimens and DST: a framework for action // Lancet Infect. Dis. – 2013. – № 13. – Р. 449-458. https://doi.org/10.1016/S1473-3099(13)70025-2Wells W.A., Boehme C.C., Cobelens F.G., Daniels C., Dowdy D., Gardiner E., Gheuens J., Kim P., Kimerling M., Kreiswirth B. et al. Alignment of new tuberculosis drug regimens and DST: a framework for action. Lancet Infect. Dis., 2013, no. 13, pp. 449-458. https://doi.org/10.1016/S1473-3099(13)70025-2World Health Organization. Global Tuberculosis Report 2024. Geneva: World Health Organization; 2024.World Health Organization. Global tuberculosis report, 2024. Geneva, World Health Organization, 2024.World Health Organization. WHO consolidated guidelines on tuberculosis. Module 3: diagnosis. Geneva: World Health Organization; 2025.World Health Organization. WHO consolidated guidelines on tuberculosis. Module 3: diagnosis. Geneva, World Health Organization, 2025.Wu Q., Zhu Y., Zhang Y., Liu Z., Zhang M., Chen J., Wu B. Evaluation and Comparison of Laboratory Methods in Diagnosing Mycobacterium tuberculosis and Nontuberculous Mycobacteria in 3012 Sputum Samples // Clin. Respir. J. – 2025. – № 19. – Р. e70071. https://doi.org/10.1111/crj.70071Wu Q., Zhu Y., Zhang Y., Liu Z., Zhang M., Chen J., Wu B. Evaluation and comparison of laboratory methods in diagnosing Mycobacterium tuberculosis and Nontuberculous Mycobacteria in 3012 Sputum Samples. Clin. Respir. J., 2025, no. 19, pp. e70071. https://doi.org/10.1111/crj.70071Yang Z., Li J., Shen J., Cao H., Wang Y., Hu S., Du Y., Wang Y., Yan Z., Xie L., et al. Recent progress in tuberculosis diagnosis: insights into blood-based biomarkers and emerging technologies // Front. Cell. Infect. Microbiol. – 2025. – Р. 15. – Р. 1567592. https://doi.org/10.3389/fcimb.2025.1567592Yang Z., Li J., Shen J., Cao H., Wang Y., Hu S., Du Y., Wang Y., Yan Z., Xie L. et al. Recent progress in tuberculosis diagnosis: insights into blood-based biomarkers and emerging technologies. Front. Cell. Infect. Microbiol., 2025, pp. 15, pp. 1567592. https://doi.org/10.3389/fcimb.2025.1567592Yuan L., Lin W., Zheng K., Zhu S. FRET-Based Small-Molecule Fluorescent Probes: Rational Design and Bioimaging Applications // Acc. Chem. Res. – 2013. – № 46. – Р. 1462-1473. https://doi.org/10.1021/ar300273vYuan L., Lin W., Zheng K., Zhu S. FRET-based small-molecule fluorescent probes: rational design and bioimaging applications. Acc. Chem. Res., 2013, no. 46, pp. 1462-1473. https://doi.org/10.1021/ar300273v

The authors declare that there are no conflicts of interest present.