tibljТуберкулез и болезни легкихTuberculosis and Lung Diseases2075-12302542-1506Медицинские знания и технологии10.21292/2075-1230-2015-0-2-4-11tiblj-334Research ArticleОБЗОРЫREVIEWSМетаболомические исследования в пульмонологииMetabolomic studies in pulmonologyФуринаР. Р.FurinaR. R.

врач-эндоскопист эндоскопического отделения,

424030, г. Йошкар-Ола, ул. Осипенко, д. 33

furina_raisa@mail.ruГБУ РМЭ «Республиканская клиническая больница»Russian Federation20150102201502411Copyright © Фурина Р.Р., 20152015Фурина Р.Р.Furina R.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.tibl-journal.com/jour/article/view/334

В обзоре отражены результаты применения метаболомических исследований в пульмонологии. Ключевая идея метаболомики заключается в обнаружении специфических биомаркеров в биологическом образце для диагностики заболеваний бронхов и легких. Приведены основные методы разделения и идентификации летучих органических веществ как биомаркеров (газовая хроматография, масс-спектрометрия, спектроскопия ядерного магнитного резонанса), применяемые в метаболомике. Также освещен метод твердофазной микроэкстракции, применяемой в качестве предварительной подготовки образца. Представлены результаты лабораторных исследований по поиску биомаркеров рака легких, острого респираторного дистресс-синдрома, хронической обструктивной болезни легких, муковисцедоза, хронических инфекций, туберкулеза легких. Кроме того, уделено внимание применению возможностей метаболомики в экспериментальной медицине, в том числе изучению бронхиальной астмы. Информация интересна как для теоретиков, так и для практикующих врачей.

The review shows the results of metabolomic studies in pulmonology. The key idea of metabolomics is to detect specific biomarkers in a biological sample for the diagnosis of diseases of the bronchi and lung. Main methods for the separation and identification of volatile organic substances as biomarkers (gas chromatography, mass spectrometry, and nuclear magnetic resonance spectrometry) used in metabolomics are given. A solid-phase microextraction method used to pre-prepare a sample is also covered. The results of laboratory tests for biomarkers for lung cancer, acute respiratory distress syndrome, chronic obstructive pulmonary disease, cystic fibrosis, chronic infections, and pulmonary tuberculosis are presented. In addition, emphasis is placed on the possibilities of metabolomics used in experimental medicine, including to the study of asthma. The information is of interest to both theorists and practitioners.

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The authors declare that there are no conflicts of interest present.