POSITIVE RESULTS OF TRANSPLANTATION OF MULTI-COMPONENT COMPOSITE MATERIAL CONTAINING ALLOGENEIC MESENCHYMAL STEM CELLS AFTER CYSTECTOMY IN A RABBIT
https://doi.org/10.21292/2075-1230-2017-95-6-45-50
Abstract
Of late much attention has been paid to tissue engineering by urologists. After successful testing on animals, artificial urinary bladders with self-specific cells were transplanted to humans. Our research is aimed at investigating the opportunity of using cellular technologies if no healthy self-specific material is available.
The goal of this experiment is to investigate the opportunity of using a multi-component composite material containing allogeneic cells to replace the defect of urinary wall under experimental conditions.
The standard technique was used for isolation and culturing of mesenchymal stromal stem cells from the rabbit's bone marrow. Multi-component composite material based on the polylactide matrix was inoculated by allogeneic cells and transplanted in vivo to the model of partial cystectomy. In 2.5 months the presence of labeled cells in the implantation site was confirmed by objective methods.
Keywords
allogeneic transplantation,
mesenchymal stromal cells of bone marrow,
urinary bladder,
tissue engineering
Supporting agencies: RNF grant no. 14-50-00068
About the Authors
N. V. Orlovа
St. Petersburg Research Institute of Phthisiopulmonology
Russian Federation
Russian Federation
Nadezhda V. Orlova - Researcher in Urology, Gynecology and Abdominal Surgery.
2-4, Ligovsky Ave., St. Petersburg, 191036, Phone: +7 (812) 297-89-88
T. I. Vinogrаdovа
St. Petersburg Research Institute of Phthisiopulmonology
Russian Federation
Russian Federation
Tatyana I. Vinogradova - Doctor of Medical Sciences, Professor, Head of Laboratory of Experimental Tuberculosis and New Medical Technologies.
2-4, Ligovsky Ave., St. Petersburg, 191036
N. M. Yudintsevа
Institute of Cytology of the Russian Academy of Science
Russian Federation
Russian Federation
Natalia M. Yudintseva - Candidate of Biological Sciences, Senior Researcher.
4, Tikhoretsku Ave., St. Petersburg, 194064
Yu. A. Nаsсhekinа
Institute of Cytology of the Russian Academy of Science
Russian Federation
Russian Federation
Yulia A. Naschekina - Candidate of Biological Sciences, Researcher.
4, Tikhoretsku Ave., St. Petersburg, 194064
M. G. Sheykhov
St. Petersburg Research Institute of Phthisiopulmonology
Russian Federation
Russian Federation
Magomedsadyk G. Sheykhov - Post-Graduate Student.
2-4, Ligovsky Ave., St. Petersburg, 191036, Phone: +7 (812) 297-89-88
P. K. Yablonsky
St. Petersburg Research Institute of Phthisiopulmonology; St. Petersburg University
Russian Federation
Russian Federation
Petr K. Yablonsky - Doctor of Medical Sciences, Professor, Director.
2-4, Ligovsky Ave., St. Petersburg, 191036, Phone: +7 (812) 579-25-54
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Review
For citations:
Orlovа N.V., Vinogrаdovа T.I., Yudintsevа N.M., Nаsсhekinа Yu.A., Sheykhov M.G., Yablonsky P.K. POSITIVE RESULTS OF TRANSPLANTATION OF MULTI-COMPONENT COMPOSITE MATERIAL CONTAINING ALLOGENEIC MESENCHYMAL STEM CELLS AFTER CYSTECTOMY IN A RABBIT. Tuberculosis and Lung Diseases. 2017;95(6):45-50. (In Russ.) https://doi.org/10.21292/2075-1230-2017-95-6-45-50
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