ROLE OF PYRIDOXINE HYDROCHLORIDE IN THE DEVELOPMENT OF TOLERANCE TO THE TOXIC ACTION OF ISONIAZID IN ANIMALS


https://doi.org/10.21292/2075-1230-2018-96-6-51-57

Full Text:


Abstract

The objective of the study: to assess the impact of continuous and course (intermittent) use of pyridoxine hydrochloride in combination with isoniazid on the rate of tolerance development in rats under conditions of a toxicological experiment.

Subjects and methods. Isoniazid (tablets, 0.3 g.) and the substance of pyridoxine hydrochloride (powder, 50.0 g.) were used for the experiment. Male white rats were used as experimental biological models.

Main results. The article presents data on experimental study of toxicologic tolerance of rats to the anti-tuberculosis drug of isoniazid with its administration simultaneously with pyridoxine hydrochloride. The obtained data provided the evidence about the impact of pyridoxine hydrochloride on the rate of how fast the host developed the tolerance to the toxic action of isoniazid. It has been proved that intermittent (by short courses) use of pyridoxine hydrochloride with isoniazid versus its continuous use resulted in a slower development of toxicological tolerance to isoniazid in rats. 


About the Authors

K. I. Usov
Research Institute of Biophysics by Angarsk State Technical University; Irkutsk State Medical University
Russian Federation

Candidate of Biological Sciences, Associate Professor, Head of Laboratory of Toxicological Tests and Testing Laboratory Center,

2, Partizanskaya St., Angarsk, Irkutsk Region, 665835



T. A. Guskova
M. V. Dorogov Center for Pharmaceutical Technology Transfer
Russian Federation

Doctor of Medical Sciences, Professor, Correspondent Member of the Russian Academy of Sciences, Honoured Researcher of the Russian Science, Deputy Head of All-Russian Public Organization of Toxicologists, Leading Researcher of Pre-clinical Trials Department,

11/2, Tekhnoparkovaya St., Yaroslavl, 150010



G. G. Yushkov
Research Institute of Biophysics by Angarsk State Technical University
Russian Federation

Candidate of Medical Sciences, Associate Professor, Researcher of Laboratory of Toxicological Tests and Testing Laboratory Center,

2, Partizanskaya St., Angarsk, Irkutsk Region, 665835



References

1. Glants S. Mediko-biologicheskaya statistika. [Medical biological statistics]. Moscow, Praktika Publ., 1999, pp. 81-266.

2. Kompantseva E.V., Ovcharenko L.P., Khalata А.V. Pharmacokinetic and general toxic assessment of granules of isoniazid and pyridoxine hydrochloride. Ekologiya Cheloveka, 2007, no. 2, pp. 7-10. (In Russ.)

3. Mironov A.N. Rukovodstvo po provedeniyu doklinicheskikh issledovaniy lekarstvennykh sredstv, Ch. 1. [Guidelines on pre-clinical trials of medications. Part 1]. Moscow, Grif I K Publ., 2012, pp. 804-809.

4. Rylova M.L. Metody issledovaniya khronicheskogo deystviya vrednykh faktorov sredy v eksperimente. [Methods of studying the chronic action of harmful environmental factors in the experiment]. Moscow, Meditsina Publ., 1964, pp. 33-40. (In Russ.)

5. RD 64-126-91 "Pravila doklinicheskoy otsenki bezopasnosti farmakologicheskikh sredstv (GLP)". [RD 64-126-91. Rules of pre-clinical safety assessment of pharmacological agents (GPL)]. Moscow, 1991, 115 p.

6. Usov K.I., Guskova T.А., Yushkov G.G. Development of toxic tolerance to isoniazid in the experiment. Toksikologicheskiy Vestnik, 2017, no. 2, pp. 2-11. (In Russ.)

7. Usov K.I., Guskova T.А., Yushkov G.G. Comparative experimental toxicological study of combinations of anti-tuberculosis drugs, containing and not containing pyridoxine hydrochloride. Voprosy Biologicheskoy, Meditsinskoy i Farmatsevticheskoy Khimii, 2017, vol. 20, no. 4, pp. 16-24. (In Russ.)

8. Usov K.I., Guskova T.А., Yushkov G.G. Sensitivity of animals from different age groups to isoniazid under conditions of a toxicological experiment. Toksikologicheskiy Vestnik, 2016, no. 5, pp. 36-43. (In Russ.)

9. Usov K.I., Yushkov G.G., Mashanov А.V. Xanthurenic acid as a valuable diagnostic factor in continuous use of modern anti-tuberculosis drugs (an experimental study). Biofarmatsevticheskiy Journal, 2016, vol. 6, no. 8, pp. 45-49. (In Russ.)

10. Usov K.I., Yushkov G.G., Mashanov А.V. Acute toxicity of anti-tuberculosis drugs, containing and not containing pyridoxine hydrochloride. Tuberculosis and Lung Diseases, 2014, no. 12, pp. 76-82. (In Russ.)

11. Usov K.I., Yushkov G.G., Shulgina N.А. On the study of formation of a physiological response of an animal to single action of megadose of B6 in the experiment. Med. Akademicheskiy Journal, 2010, no. 5, pp. 36-37. (In Russ.)

12. Khabriev R.U. Rukovodstvo po eksperimentalnomu (doklinicheskomu) izucheniyu novykh farmatsevticheskikh veschestv. [Manual on the experimental (pre-clinical) study of new pharmaceutical substances]. Moscow, Meditsina Publ., 2005, pp. 244-253.

13. http://www.medicinform.net/tuberculos/book/tb_book9.htm.

14. http://www.docs.cntd.ru/document/456016716. 15. http://www.docs.cntd.ru/document/420350679.


Supplementary files

For citation: Usov K.I., Guskova T.A., Yushkov G.G. ROLE OF PYRIDOXINE HYDROCHLORIDE IN THE DEVELOPMENT OF TOLERANCE TO THE TOXIC ACTION OF ISONIAZID IN ANIMALS. Tuberculosis and Lung Diseases. 2018;96(6):51-57. https://doi.org/10.21292/2075-1230-2018-96-6-51-57

Views: 614

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2075-1230 (Print)
ISSN 2542-1506 (Online)