The prevalence, clinical and epidemiological significance of rare genotypes of M. tuberculosis isolated from tuberculosis patients in Saratov Region
https://doi.org/10.21292/2075-1230-2020-98-2-5-9
Abstract
The objective: to identify the role, clinical and epidemiological significance of rare genotypes of M. tuberculosis (MTB) in the epidemiology of tuberculosis in Saratov Region.
Materials. The spoligotyping and hybridization method by the Spoligo-biochip kit (BIOCHIP-IMB OOO, Moscow) was used to examine sputum samples of 353 patients with newly diagnosed pulmonary TB, who were permanent residents of 20 cities and districts of Saratov Region.
Results. MTB population circulating in the examined TB patients was heterogeneous and represented by 13 genotypes (Beijing, Beijing-like, Haarlem, Ural, LAM, T, Manu, Microti, Rus 1, Cas, EAI, Canetti, AFRI). Rare genotypes (EAI, Manu, Microti, Rus 1, Cas, AFRI, Canetti) were found in 42/353 (11.9%) patients, of which EAI genotype was found in 8 patients (2.3%), Manu in 14 ( 3.9%), Microti - 13 (3.7%), Cas - 2 (0.57%), Rus 1 - 1 (0.28%), Canetti - 3 (0.85%) , and AFRI - in 1 (0.28%). Tuberculosis cases caused by the Microti genotype were endemic and characterized by homogeneity of the revealed spoligotypes (SIT 539). Isolates of the Manu genotype were registered only in men (100%), mainly living in rural areas (71.4%) and were characterized by a high heterogeneity of SIT (1195, 1485, 1460, 1482, 1088). Tuberculosis caused by the EAI genotype was characterized by a smaller number of destructive forms and forms with bacterial excretion, and high SIT heterogeneity (300, 1431, 1428, 924, 622, 192). There were single cases of the Cas, Canetti, and R genotypes.
About the Authors
T. Yu. Salina
V.I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Doctor of Medical Sciences, Associate Professor, Professor of Phthisiology Department
Phone: + 7 (8452) 26-56-08
112, B. Kazachya St., Saratov, 410012
T. I. Morozova
V.I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Doctor of Medical Sciences, Professor, Head of Phthisiology Department
Phone/Fax: + 7 (8452) 26-16-90
112, B. Kazachya St., Saratov, 410012
References
1. Vasilenko N.V., Budritskiy А.M. Contemporary views on genetic families of M. tuberculosis. Vestnik VGMU, 2014, vol. 13, no. 5, pp. 16-22. (In Russ.)
2. Limanskaya O.Yu. Species-specific detection of mycobacterium tuberculosis complex. Tuberculosis and Lung Diseases, 2009, vol. 86, no. 10, pp. 49-55. (In Russ.)
3. Prozorov А.А., Danilenko V.N. Mycobacteria of tuberculous complex: genomics, molecular epidemiology, ways of evolution. Uspekhi Sovremennoy Biologii, 2011, vol. 131, no. 3, pp. 227-243. (In Russ.)
4. Savilov E.D., Sinkov V.V., Ogarkov O.B. Epidemiologiya tuberkuleza na Evro-Aziatskom kontinente: otsenka globalnogo dvizheniya shtammov genotipa «Pekin» – Irkutsk. [Epidemiology of tuberculosis in Euro-Asian Continent: evaluation of global movement of strains of Beijing genotype – Irkutsk]. RIO GBOU DPO IGMAPO Publ., 2013, 120 p.
5. Shitikov E.А. Genomnaya variabelnost vozbuditeley lekarstvenno-ustoychivogo tuberkuleza, rasprostranennykh na territorii Rossiyskoy Federatsii. Diss. kand. biol. nauk. [Genomic complexity of drug resistant tuberculosis macybacteria prevalent on the territory of the Russian Federation. Cand. Diss.]. Moscow, 2014, 123 p.
6. Shulgina M.V., Narvskaya O.V., Mokrousov I.V., Vasilyeva I.А. Patogennye i uslovno-patogennye mikobakterii. [Pathogenic and opportunistic pathogenic mycobacteria]. Moscow, New Terra Publ., 2018, 104 p.
7. Maguga-Phasha N.T.C., Manyai N.S., Mashinya F., Makgatho M.E., Mbajiorgu E.F. Cenetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa. BMC Infect Dis., 2017, vol. 17, no. 1, pp. 764. doi: 10.1186/s12879-017-2881-z. PubMed PMID: 2 8. 9233106; PubMed Central PMCID: PMC5727936.
8. Michelet L., de Cruz К., Zanella G., Aaziz R., Bulach T., Karoui C., Henault S., Jouncour G, Boschiroli M.L. Infection with Micobacterium microti in Animals in France. J. Clin. Microbiol., 2015, vol. 53, no. 3, pp. 981-985.
9. Panteix G., Gutierrez M.C., Boschiroli M.L., Rouviere M., Plaidy A., Pressac D., Porcheret H., Chyderiotis G., Ponsada M., Van Oortegem K., Salloum S., Cabuzel S., Bañuls A.L., Van de Perre P., Godreuil S. Pulmonary tuberculosis due to Mycobacterium microti: a study of six recent cases in France. J. Med. Microbiol., 2010, no. 59, pp. 984-989.
10. Singh U.B., Suresh N., Bhanu N.V., Arora J., Pant H., Sinha S., Aggarwal R. C., Singh S., Pande J.N., Sola C., Rastogi N., Seth P. Predominant Tuberculosis Spoligotypes, Delhi, India. Emerg. Infect. Dis., 2004, vol. 10, no. 6, pp. 1138-1142.
Review
For citations:
Salina T.Yu., Morozova T.I. The prevalence, clinical and epidemiological significance of rare genotypes of M. tuberculosis isolated from tuberculosis patients in Saratov Region. Tuberculosis and Lung Diseases. 2020;98(2):5-9. (In Russ.) https://doi.org/10.21292/2075-1230-2020-98-2-5-9
Views:
778
Email this article 