The optimal modeling method of specific tuberculosis peritonitis (experimental research)
D. V. Plotkin,
T. I. Vinogradova,
M. N. Reshetnikov,
Yu. R. Zyuzya,
M. S. Serdobintsev,
M. N. Sinitsyn https://doi.org/10.21292/2075-1230-2021-99-12-44-50
Abstract
The objective: to create a reproducible model of chronic tuberculosis peritonitis to study pathophysiological mechanisms of its progression and to develop pathogenetically based therapy.
Subjects and Methods. The study was performed using 10 male rabbits of the Chinchilla breed. The animals were administered intraperitoneal culture of Mycobacterium tuberculosis, tuberculosis peritonitis modeling was performed according to the proposed method.
Results. In the course of the experiment, it was proved that all animals developed tuberculous peritonitis with lesions of the large omentum and serous integuments of internal organs. Molecular genetic tests of fragments of the omentum and peritoneum detected DNA of Mycobacterium tuberculosis.
About the Authors
D. V. Plotkin
Moscow Municipal Scientific Practical Center of Tuberculosis Control; Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
Dmitry V. Plotkin, Candidate of Medical Sciences, Surgeon of Tuberculosis Surgery Department no. 2.
10, Stromynka St., Moscow, 107014.
Phone: +7 (499) 268-28-10.
T. I. Vinogradova
St. Petersburg Research Institute of Phthisiopulmonology
Russian Federation
Russian Federation
Tatyana I. Vinogradova, Doctor of Medical Sciences, Professor, Chief Researcher, Head of Experimental Tuberculosis Laboratory.
2-4, Ligovsky Ave., St. Petersburg, 191036
Phone: +7 (812) 775-75-55
M. N. Reshetnikov
Moscow Municipal Scientific Practical Center of Tuberculosis Control
Russian Federation
Russian Federation
Mikhail N. Reshetnikov, Candidate of Medical Sciences, Surgeon of Tuberculosis Surgery Department Clinic no. 2.
10, Stromynka St., Moscow, 107014.
Phone: +7 (499) 268-28-10.
Yu. R. Zyuzya
Moscow Municipal Scientific Practical Center of Tuberculosis Control
Russian Federation
Russian Federation
Yuliya R. Zyuzya, Candidate of Medical Sciences, Leading Researcher of Department of Laboratory Diagnostics of Tuberculosis and Pathomorphology. Pathologist.
10, Stromynka St., Moscow, 107014.
Phone: +7 (499) 268-28-10.
M. S. Serdobintsev
St. Petersburg Research Institute of Phthisiopulmonology
Russian Federation
Russian Federation
Mikhail S. Serdobintsev, Doctor of Medical Sciences, Professor, Leading Researcher.
2-4, Ligovsky Ave., St. Petersburg, 191036
Phone: +7 (812) 297-84-53.
M. N. Sinitsyn
Moscow Municipal Scientific Practical Center of Tuberculosis Control
Russian Federation
Russian Federation
Mikhail V. Sinitsyn, Doctor of Medical Sciences, Deputy Director.
10, Stromynka St., Moscow, 107014.
Phone: +7 (499) 268-28-10.
References
1. Bogorodskaya E.M., Sinitsyn M.V., Belilovsky E.M., Borisov S.E., Kotova E.А. Impact of HIV infection on the structure of new tuberculosis cases detected in the city of Moscow Tuberculosis and Lung Diseases, 2017, vol. 95, no. 10, pp. 17-26. (In Russ.) https://doi.org/10.21292/2075-1230-2017-95-10-17-26.
2. GOST 33216-2014. Rules of work with laboratory rodents and rabbits. Moscow, Standartinform Publ., 2016. (In Russ.)
3. Zuban O.N., Belilovsky E.M., Grabarnik А.E., Chotchaev R.M., Rodchenkov А.V. Epidemiology of extrapulmonary tuberculosis in a big city. Tuberculosis and Lung Diseases, 2019, vol. 97, no. 12, pp. 55-56. (In Russ.) https://doi.org/10.21292/2075-1230-2019-97-12-55-56.
4. Naymanov А.Kh., Gulyukin А.M., Tolstenko N.G., Vangeli E.P., Kalmykov V.M. Diaskintest for the diagnosis of bovine tuberculosis. Tuberculosis and Lung Diseases, 2020, vol. 98, no. 12, pp. 53-56. (In Russ.) http://doi.org/10.21292/2075-1230-2020-98-12-53-56.
5. Naymanov А.Kh., Kalmykov V.M., Kalmykova M.S. Reproduction of tuberculosis on laboratory animals (biological test). Veterinariya, Zootekhniya I Biotekhnologiya, 2018, no. 5, pp. 24-30. (In Russ.)
6. Saveliev V.S., Gelfand B.R., Filimonov M.I., Podachin P.V., Sergeeva N.А. Criteria for selecting an effective surgical treatment tactic for dissemonated peritonitis. Annaly Khirurgii, 2013, vol. 18, no. 2, pp. 48-54. (In Russ.)
7. Savonenkova L.N., Аryamkina O.L. Pathogenesis and morphogenesis of abdominal tuberculosis with involvement of the gastrointestinal tract. Nizhegorodskiy Meditsinskiy Journal, 2006, no. 2, pp. 89-94. (In Russ.)
8. Titarenko O.T., Dyakova M.E., Perova T.L., Ryasnyanskaya T.B. Activity of adenosine deaminase and its isozymes in patients with various forms of tuberculosis. Probl. Tub., 2002, vol. 81, no. 3, pp. 43-45. (In Russ.)
9. Tomnyuk N.D., Danilina E.P., Chernykh А.N., Parno А.А., Shurko K.S. Peritonitis as one of the main causes of a fatal outcome. Sovremennye Naukoemkie Tekhnologii, 2010, no. 10, pp. 81-84. (In Russ.)
10. Chepurnykh E.E., Shurygina I.А., Fadeeva T.V., Grigoriev E.G. Experimental modeling of general purulent peritonitis. Acta Biomedica Scientifica (East Siberian Biomedical Journal), 2019, vol. 4, no. 3, pp. 117-121. (In Russ.) https://doi.org/10.29413/ABS.2019-4.3.15.
11. Shekunova E.V., Kovaleva M.А., Makarova M.N., Makarov V.G. Dose selection in preclinical studies: cross-species dose conversion. The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products, 2020, vol. 10, no. 1, pp. 19-28. (In Russ.) https://doi.org/10.30895/1991-2919-2020-10-1-19-28.
12. Shepelkova G.S., Evstifeev V.V., Аpt А.S. The study of molecular mechanisms of tuberculosis pathogenesis in experimental models. Tuberculosis and Lung Diseases, 2012, vol. 89, no. 7, pp. 3-11. (In Russ.)
13. Chong V.H. Challenges with extrapulmonary tuberculosis. South Med. J., 2009, vol. 102, no. 5, pp. 551-522. https://doi.org/10.1097/SMJ.0b013e3181a09331.
14. Cooper D., Yao V., McCauley R., Hall J., Platell C. A low-morbidity murine model of peritonitis. Dis. Colon. Rectum., 2002, vol. 45, no. 3, pp. 394-400. https://doi.org/10.1007/s10350-004-6189-x.
15. Fell L.H., Seiler-Mußler S., Sellier A.B., Rotter B., Winter P., Sester M. et al. Impact of individual intravenous iron preparations on the differentiation of monocytes towards macrophages and dendritic cells. Nephrol. Dial. Transplant., 2016, vol. 31, no. 11, pp. 1835-1845. https://doi.org/10.1093/ndt/gfw045.
16. Hamdi H., Mariette X., Godot V., Weldingh K., Hamid A.M., Prejean M.V. et al. Inhibition of anti-tuberculosis T-lymphocyte function with tumour necrosis factor antagonists. Arthritis Res. Ther., 2006, vol. 8, no. 4, pp. R114. https://doi.org/10.1186/ar1994.
17. Hillebrand D.J., Runyon B.A., Yasmineh W.G., Rynders G.P. Ascitic fluid adenosine deaminase insensitivity in detecting tuberculous peritonitis in the United States. Hepatology, 1996, vol. 24, no. 6, pp. 1408-1412. https://doi.org/10.1002/hep.510240617.
18. Nairz M., Theurl I., Swirski F.K., Weiss G. "Pumping iron" ‒ how macrophages handle iron at the systemic, microenvironmental, and cellular levels. Pflugers Arch., 2017, vol. 469, no. 3-4, pp. 397-418. https://doi.org/10.1007/s00424-017-1944-8.
19. Omachi K. Experimental tuberculous infection in mice. Tohoku J. Exp. Med., 1953, vol. 57, no. 4, pp. 317-326. https://doi.org/10.1620/tjem.57.317.
20. Salgado Júnior W., Santos J.S., Cunha F. de Q. Development of a lethal model of peritonitis for assessment of laparoscopic and laparotomic treatments in rats. Acta Cir. Bras., 2007, vol. 22, no. 1, pp. 39-42. https://doi.org/10.1590/s0102-86502007000100007.
21. Saliu O.Y., Sofer C., Stein D.S., Schwander S.K., Wallis R.S. Tumor-necrosis-factor blockers: differential effects on mycobacterial immunity. J. Infect. Dis., 2006, vol. 194, no. 4, pp. 486-492. https://doi.org/10.1086/505430.
22. Singhal A., Aliouat E.M., Hervé M., Mathys V., Kiass M., Creusy C., Delaire B. et al. Experimental tuberculosis in the Wistar rat: a model for protective immunity and control of infection. PLoS One, 2011, vol. 6, no. 4, pp. e18632. https://doi.org/10.1371/journal.pone.0018632.
23. Srivastava U., Almusa O., Tung K.W., Heller M.T. Tuberculous peritonitis. Radiol. Case Rep., 2015, vol. 9, no. 3, pp. 971. https://doi.org/10.2484/rcr.v9i3.971.
24. Sukhbaatar N., Weichhart T. Iron regulation: macrophages in control. Pharmaceuticals (Basel), 2018, vol. 11, no. 4, pp. 137. https://doi.org/10.3390/ph11040137.
Review
For citations:
Plotkin D.V., Vinogradova T.I., Reshetnikov M.N., Zyuzya Yu.R., Serdobintsev M.S., Sinitsyn M.N. The optimal modeling method of specific tuberculosis peritonitis (experimental research). Tuberculosis and Lung Diseases. 2021;99(12):44-50. (In Russ.) https://doi.org/10.21292/2075-1230-2021-99-12-44-50
Views:
437
Email this article 