Oxygen-Dependent Blood Processes in Beck's Sarcoidosis

The objective: to assess the state of oxygen-dependent blood processes in patients with Beck's sarcoidosis.

Subjects and Methods. Parameters of blood oxygen transport function and free radical lipid oxidation, as well as the content of nitrogen monoxide and hydrogen sulfide gas transmitters were assessed in the blood of 75 patients (41 women; 34 men) with Beck's sarcoidosis, stage 2 (the pulmonary mediastinal form).

Results. In the pulmonary mediastinal form of sarcoidosis versus healthy individuals, there was a decrease in the degree of blood oxygen saturation from 65.4 (60.6; 67.8) to 41.50 (36.0; 49.8), p < 0.05, %, partial oxygen pressure from 40.0 (38.0; 47.0) to 23.0 (18.3; 29.0) mmHg, p < 0.05, and blood pH increased. A decrease in the index of hemoglobin affinity to oxygen p_50real (26.80 (24.70; 31.40), p < 0.05, versus healthy individuals 28.2 (27.9; 29.1) mmHg) was revealed. The level of diene conjugates increased in plasma by 86.4% (p < 0.05) and malondialdehyde increased in erythrocytes by 139.2% (p < 0.05), while the following parameters decreased: α-tocopherol (by 42.7 %, p < 0.05), retinol (by 41.9%, p < 0.05), reduced glutathione (by 30.4%, p < 0.05), and ceruloplasmin (by 22.3%, p < 0.05) in plasma. There was an increase in the concentration of nitrogen monoxide (from 15.42 (14.48; 17.71) to 17.09 (7.04; 25.09), mmol/L) and a decrease in hydrogen sulfide (from 29.01 (25.21; 37.03) to 13.08 (8.63; 20.46), mmol/L) in the blood in case of this pathology.

Conclusion. There is a deterioration in the blood oxygen-binding properties in patients with Beck's sarcoidosis, stage 2, and as a consequence, tissue needs in oxygen are not adequately provided by blood circulation. There is an increase in the concentration of nitrogen monoxide and a decrease in hydrogen sulfide, which affects the oxygen transport function of blood which may be important for ensuring the processes of oxygen transfer to tissues. In this pathology, activation of lipid peroxidation and a decrease in the level of antioxidant protection factors of blood were revealed which might be important for the genesis of this disease.

1. Borisov V.B., Forte E. Terminal oxidase cytochrome bd protects bacteria from the toxic effects of hydrogen sulfide. Biokhimiya, 2021, vol. 86, issue 1, pp. 30-42. (In Russ.)

2. Vasiliev A.G. et al. On the pathogenesis of acute blood loss syndrome. Pediatr, 2019, vol. 10, no. 3, pp. 93-100. (In Russ.)

3. Vizel A.A., Gorblyansky Yu.Yu., Ilkovich M.M. et al. Fibrosing sarcoidosis: from understanding to treatment perspective. Prakticheskaya Pulmonologiya, 2021, no. 1, pp. 61-73. (In Russ.)

4. Vyushina A.V., Ordyan N.E. Some aspects of the current state of prenatal stress and the role of oxidative stress in the realisation of its consequences. Uspekhi Sovremennoy Biologii, 2021, vol. 141, no. 2, pp. 133-148. (In Russ.)

5. Zinchuk V.V. Oxygen transport functions of blood and hydrogen sulfide gazotransmitter. Uspekhi Fiziologicheskikh Nauk, 2021, vol. 52, no. 3, pp. 41-55. (In Russ.)

6. Kamyshnikov V.S. Spravochnik po kliniko-biokhimicheskoy laboratornoy diagnostike. [Handbook on clinical biochemical diagnostics]. Moscow, MEDpress-Inform Publ., 2009, 889 p.

7. Kengaiah J., Nandish S.K.M., Ramachandraiah Ch. Protective efficacy of tamarind seed coat ethanolic extract on oxidative stress induced eryptosis. Biokhimiya, 2020, vol. 85, no. 1, pp. 139-152. (In Russ.)

8. Korolyuk M.A. et al. Technique for testing the catalase activity. Lab. Delo, 1988, no. 1, pp. 16-19. (In Russ.)

9. Mikashinovich Z.I., Vinogradova E.V., Belousova E.S. Effect of statins (Zocor) on oxygen-dependent processes in muscle tissue and erythrocytes in animals with hypercholesterolemia. Acta Biomedica Scientifica, 2019, no. 3, pp. 110-116. (In Russ.)

10. Muravyov A.V. The role of gaseous mediators (CO, NO and H2S) in the regulation of blood circulation: analysis of the participation of blood cell microrheology. Regionarnoe Krovoobraschenie i Mikrotsirkulyatsiya, 2021, vol. 20, no. 1, pp. 91-99. (In Russ.)

11. Nikolaev A.V., Fedotkina T.V., Churilov L.P. Interaction of inflammation, stress and acute phase response in pulmonary granulomatosis: hyperprocalcitoninemia in sarcoidosis. Rossiyskie Biomeditsinskie Issledovaniya, 2021, vol. 6, no. 1, pp. 3-12. (In Russ.)

12. Sidorenko I.A., Krishneva S.A., Khokhlova A.V. Changes in the indicators of blood gas transport function in patients with acute ischemic stroke. Nauchny Almanakh, 2019, no. 3, pp. 174-176. (In Russ.)

13. Tiemann K., Schneckenburger J., Shik V. et al. Oxidative stress and NO generation in cerulein-induced acute pancreatitis in rats. Journal Anatomii i Gistopatologii, 2019, vol. 8, no. 1, pp. 68-76. (In Russ.)

14. Frantsuzevich L.Ya., Bobkov A.P., Dzhain M. et al. Phenotyping of angiotensin-Iconverting enzyme in patients with systemic manifestations of sarcoidosis. Lechebnoye Delo, 2021, no. 2, pp. 78-87. (In Russ.)

15. Bryan N.S., Grisham M.B. Methods to detect nitric oxide and its metabolites in biological samples. Free Radic. Biol. Med., 2007, vol. 43, no. 5, pp. 645-657.

16. Norris E.J. et al. The liver as a central regulator of hydrogen sulfide. Shock, 2011, vol. 36, no. 3, pp. 242-250.

17. Sedlak J., Lindsay R.N. Estimation of total, protein-bound, and protein sulfhydryl groups in tussue with Ellman’s reagent. Anal. Biochem., 1968, vol. 25, no. 1, pp. 192-205.

18. Severinghaus J.W. Blood gas calculator. J. Appl. Physiol., 1966, vol. 21, no. 3, pp. 1108-1116.

19. Storz J.F. Hemoglobin-oxygen affinity in high-altitude vertebrates: is there evidence for an adaptive trend? J. Exp. Biol., 2016, vol. 219, no. 20, pp. 3190-3203.

20. Taylor S.L., Lamden M.P., Tappel A.L. Sensitive fluorometric method for tissue tocopherol analysis. Lipids, 1976, vol. 11, no. 7, pp. 530-538.