Morphological and Functional Characteristics of the Microenvironment After Pleural Implantation of Biosynthetic Materials
https://doi.org/10.58838/2075-1230-2023-101-1-48-54
Abstract
The objective: to study morphological changes in tissues after implantation of a bioresorbable material synthesized on the basis of polylactide (PLA) and polycaprolactone (PCL) in various ratios.
Subjects and Methods. Laboratory animals Chinchilla lanigera (n = 12) underwent intrapleural implantation with 2% PLA/PCL 1/1 and 4% PLA/PCL 1/3 materials. Morphometric assessment was carried out in14 and 28 weeks, the animals were withdrawn from the experiment by an anesthetic overdosing.
Results. When studying tissues adjacent to the implant regardless of differences in its composition, similar morphological signs were observed. A fibrous capsule formed around the implants, and it was more pronounced in 4% of PLA/PCL 1/3. In the structure of the implants, we noted the growth of loose and dense unformed fibrous connective tissue with blood vessels with a significant replacement of the bioresorbable polymer material (by 2/3). Lymphocytes, plasma cells, single macrophages, and multinucleated cells were predominantly located at the implant/tissue interface. In all studied samples, no necrosis was observed in the implantation bed and surrounding tissue.
Conclusion. The data obtained confirm the biosafety of biomaterials and demonstrate the potential for their use in surgical treatment of humans.
About the Authors
G. A. Demyashkin
I.M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
Grigoriy A. Demyashkin, Candidate of Medical Sciences, Head of Histology and Immunohistochemistry Laboratory
Moscow
T. K. Tokaev
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Timur K. Tokaev, Researcher of Pulmonary Tuberculosis Surgery Department
Build. 2, 4, Dostoevskiy St., Moscow, 127473
A. S. Bikbaev
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Aleksandr S. Bikbaev, Thoracic Surgeon
Build. 2, 4, Dostoevskiy St., Moscow, 127473
M. V. Sinitsyn
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health; Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
Mikhail V. Sinitsyn, Doctor of Medical Sciences, Deputy Head Physician for Surgery, Professor of Phthisiology Department
Build. 2, 4, Dostoevskiy St., Moscow, 127473
T. E. Grigoryev
Kurchatov Institute National Research Center
Russian Federation
Russian Federation
Timofey E. Grigoryev, Candidate of Physical and Mathematical Sciences, Deputy Head for Research of Kurchatov Unit of Nano-, Bio-, Information, Cognitive, Socio-Humanitarian Sciences
1, Akademika Kurchatova Sq., Moscow, 123182
Yu. D. Zagoskin
Kurchatov Institute National Research Center
Russian Federation
Russian Federation
Yury D. Zagoskin, Candidate of Chemical Sciences, Researcher of Polymer Laboratory Material, Kurchatov Unit of Nano-, Bio, Information, Cognitive, Socio-Humanitarian Sciences, Research Center
1, Akademika Kurchatova Sq., Moscow, 123182
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Review
For citations:
Demyashkin G.A., Tokaev T.K., Bikbaev A.S., Sinitsyn M.V., Grigoryev T.E., Zagoskin Yu.D. Morphological and Functional Characteristics of the Microenvironment After Pleural Implantation of Biosynthetic Materials. Tuberculosis and Lung Diseases. 2023;101(1):48-54. (In Russ.) https://doi.org/10.58838/2075-1230-2023-101-1-48-54
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