Immunobiological Methods for Monitoring Latent Tuberculosis Infection in Children and Adolescents and Analysis of Tuberculosis Infection Reactivation
https://doi.org/10.58838/2075-1230-2024-102-6-32-38
Abstract
The objective: to develop a new method to detect early subclinical signs of LTBI progression to the active disease.
Subjects and Methods. Children under 18 years of age were enrolled in the study, of them 8 children suffered from pulmonary tuberculosis (TB Group) and 91 had LTBI (LTBI Group). Detection of subclinical signs of tuberculosis infection activity in LTBI Group included testing the concentration of antigen-specific induction of IFN-γ at the level more than 14 pg/ml, IL-6 more than 8 pg/ml and positive values of threshold cycles (Ct) of mRNA samples based on the results of real-time PCR of genes: PDCD1, PDL2, BATF2 and GBP5.
Results. The developed method for detection of subclinical signs of tuberculosis infection activity in children and adolescents with LTBI consists of two components (immunological testing of concentration of IFN-γ and IL-6; and molecular genetic testing of mRNA expression of the PDL2, BATF2 and GBP5 genes). Simultaneous registration of IFN-γ and IL-6 levels and mRNA expression of the PDL2, BATF2 and GBP5 genes above reference values indicates a high risk of developing active tuberculosis. It was observed in 29/91 (31.9%) patients with LTBI, in whom chest CT revealed calcifications in the upper lymph nodes or pulmonary lesions within 1 year.
About the Authors
M. A. Vladimirskiy
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Mikhail A. Vladimirskiy - Doctor of Medical Sciences, Professor, Head of Research Laboratory of Immunopathology and Immunodiagnostics of Tuberculosis Infection
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: + 7 (495) 681-11-66
M. B. Lapenkova
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Marina B. Lapenkova - Candidate of Medical Sciences, Researcher of Laboratory of Immunopathology and Immunodiagnostics of Tuberculosis Infection
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: + 7 (495) 681-11-66
A. A. Elov
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Andrey A. Elov - Doctor of Biological Sciences, Leading Researcher of Laboratory of Immunopathology and Immunodiagnostics of Tuberculosis Infection
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: + 7 (495) 681-11-66
V. A. Aksenova
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Valentina A. Aksenova - Doctor of Medical Sciences, Professor
M. A. Plekhanova
Moscow Regional Clinical TB Dispensary
Russian Federation
Russian Federation
Maria A. Plekhanova - Doctor of Medical Sciences, Associate Professor, Deputy Head Physician for Research and Clinical Activities
1 Bd. 1 Borby Sq., Moscow, 127030
Phone: +7 (496) 588-41-29
S. V. Smerdin
Moscow Regional Clinical TB Dispensary
Russian Federation
Russian Federation
Sergey V. Smerdin - Doctor of Medical Sciences, Professor, Honored Doctor of Russia, Head Physician
1 Bd. 1 Borby Sq., Moscow, 127030
Phone: +7 (496) 588-41-29
P. N. Rutkevich
E.I. Chazov National Medical Research Center of Cardiology, Russian Ministry of Health
Russian Federation
Russian Federation
Pavel N. Rutkevich - Researcher of the Laboratory, V.N. Smirnov Research Institute of Experimental Cardiology
5A Ak. Chazov St., Moscow, 121552
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Review
For citations:
Vladimirskiy M.A., Lapenkova M.B., Elov A.A., Aksenova V.A., Plekhanova M.A., Smerdin S.V., Rutkevich P.N. Immunobiological Methods for Monitoring Latent Tuberculosis Infection in Children and Adolescents and Analysis of Tuberculosis Infection Reactivation. Tuberculosis and Lung Diseases. 2024;102(6):32-38. (In Russ.) https://doi.org/10.58838/2075-1230-2024-102-6-32-38
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