Possibilities of Using Trehalose Probes for Detection of Mycobacterium tuberculosis
O. A. Ambartsumyan,
P. I. Eliseev,
O. A. Skuredina,
E. Yu. Gosteva, 
A. G. Samoylova,
I. A. Vasilyeva
A. G. Samoylova,
I. A. Vasilyeva https://doi.org/10.58838/2075-1230-2025-103-6-96-103
Abstract
Trehalose probes seem to be a promising area of tuberculosis diagnosis, these probes are capable of selectively penetrating Mycobacterium tuberculosis. These probes generate a fluorescent signal, enabling detection of mycobacteria. To analyze the current state of knowledge and prospects of trehalose probes as a new approach for rapid detection of Mycobacterium tuberculosis, a systematic review of scientific literature was conducted. The main types of probes include fluorogenic probes, "fluorophore-quencher" based probes, and photoactivatable probes. Trehalose probes enable selective detection of mycobacteria due to specific trehalose uptake and incorporation into the cell wall, followed by fluorescence activation. These probes allow for the detection of mycobacteria in sputum samples without complex sample preparation or washing. The method allows differentiation of viable and non-viable cells and can also be applied for drug susceptibility testing.
About the Authors
O. A. Ambartsumyan
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health; Osipyan Institute of Solid State Physics, Russian Academy of Sciences
Russian Federation
Russian Federation
Oganes A. Ambartsumyan - Junior Researcher of Research Microbiology Laboratory, Researcher of Laboratory of Nonequilibrium Electronic Processes
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: +7 (495) 631-15-15
P. I. Eliseev
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Platon I. Eliseev - Candidate of Medical Sciences, Head of Microbiology Research Laboratory
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: +7 (495) 631-15-15
O. A. Skuredina
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Olesya A. Skuredina - Researcher of Microbiology Research Laboratory
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: +7 (495) 631-15-15
E. Yu. Gosteva
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Ekaterina Yu. Gosteva - Junior Researcher of Microbiology Research Laboratory
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: +7 (495) 631-15-15
A. G. Samoylova
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Anastasiya G. Samoylova - Doctor of Medical Sciences, Deputy Director for Research
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: +7 (495) 631-15-15
I. A. Vasilyeva
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Irina A. Vasilyeva - Doctor of Medical Sciences, Professor, Director, Head of Phthisiology Department, Clinical Medicine Institute, Pirogov Russian National Research Medical University, Russian Ministry of Health
Build. 2, 4 Dostoevskiy St., Moscow, 127473
Phone: +7 (495) 631-15-15
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For citations:
Ambartsumyan O.A., Eliseev P.I., Skuredina O.A., Gosteva E.Yu., Samoylova A.G., Vasilyeva I.A. Possibilities of Using Trehalose Probes for Detection of Mycobacterium tuberculosis. Tuberculosis and Lung Diseases. 2025;103(6):96-103. (In Russ.) https://doi.org/10.58838/2075-1230-2025-103-6-96-103
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