PRODUCTION OF MACROPHAGE AND INTERLEUKIN-8 MIGRATION INHIBITION FACTOR IN CHILDREN AND ADOLESCENTS SUFFERING FROM DESTRUCTIVE PULMONARY TUBERCULOSIS
https://doi.org/10.21292/2075-1230-2015-0-8-43-47
Abstract
The goal of the study is to assess the changes in spontaneous and antigen-induced production of macrophages and interleukin-8 in destructive tuberculosis patients during the intensive phase of treatment. The cultures of whole blood of 38 destructive tuberculosis patients were used as a source of macrophages and interleukin-8 production, and the patients were divided depending on procalcitonin semiquantitative test (BRAHMS PCT-Q) results into the group of PCT+ (15 patients) and the group of PCT- (23 patients). Production of macrophages and interleukin-8 were assessed in the cultured blood for 20-22 hours. Antigen-induced synthesis of macrophages was assessed after adding 20 mkg/ml of PPD or 1 mkg/ml of ESAT 6/CFP 10. Production of cytokines was tested by enzyme immunoassay. It has been found that the rates of spontaneous production of macrophages or interleukin-8 in the cultured blood reflect the changes in the production of these factors and can be used without consideration of antigen-induced synthesis; spontaneous production of macrophages in patients with positive test for PCT confidently decreases and production of IL-8 confidently goes up by the 2nd month of anti-tuberculosis chemotherapy; changes in spontaneous production of macrophages di}er in patients with the various course of destructive tuberculosis (favorable, torpid, progressing). In the favorable course of the disease production of macrophages increases, in torpid or progressing ones – goes down or stays unchanged.
About the Authors
M. M. Averbakh (Jr.)
Central Tuberculosis Research Institute
Russian Federation
Moscow
Russian Federation
Moscow
L. V. Panova
Central Tuberculosis Research Institute
Russian Federation
Moscow
Russian Federation
Moscow
E. S. Ovsyankina
Central Tuberculosis Research Institute
Russian Federation
Moscow
Russian Federation
Moscow
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Review
For citations:
Averbakh (Jr.) M.M., Panova L.V., Ovsyankina E.S. PRODUCTION OF MACROPHAGE AND INTERLEUKIN-8 MIGRATION INHIBITION FACTOR IN CHILDREN AND ADOLESCENTS SUFFERING FROM DESTRUCTIVE PULMONARY TUBERCULOSIS. Tuberculosis and Lung Diseases. 2015;(8):43-47. (In Russ.) https://doi.org/10.21292/2075-1230-2015-0-8-43-47
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