MOLECULAR GENETIC ANALYSIS AND SPECTRUM OF MUTATIONS IN KATG, INHA, RPOB GENES CODING DRUG RESISTANCE TO ISONIAZID AND RIFAMPICIN IN THOSE SUFFERING FROM TB/HIV CO-INFECTION
https://doi.org/10.21292/2075-1230-2016-94-8-54-59
Abstract
51 patients suffering from pulmonary tuberculosis with concurrent HIV infection (Group 1) had PCR testing in real time (ZAO Sintol, Moscow) of sputum samples in order to study the spectrum and prevalence of mutations in katG, inhA, rpoB genes coding drug resistance (DR) to isoniazid (H) and rifampicin (R). The control group included 70 HIV negative tuberculosis patients (Group 2). No significant differences in the number and spectrum of mutations in genes coding DR to H and R were found between two groups. However in Group 1 among clinical isolates with multiple drug resistance the confident prevalence of combination of mutations (rpoB Ser531- > Leu + katG Ser315- > Thr1) was found compared to Group 2 where the combined mutations of rpoB Ser531 > Leu + katG Ser315- > Thr1 + inhA 15 were registered more often where mutations in rpoB gene were combined with mutations simultaneously in two genes coding DR to H (katG + inhA).
About the Authors
T. Yu. Salina
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Doctor of Medical Sciences, Associate Professor at FPk and PPS tuberculosis Control Department,
112, B. Kazachya St., Saratov, 410012
S. A. Churkin
Orenburg Regional Clinical TB Dispensary
Russian Federation
Russian Federation
Candidate of Medical Sciences, Head Doctor,
6, Nezhinskoye rd, Orenburg, 460041
T. I. Morozova
V. I. Razumovsky Saratov State Medical University
Russian Federation
Russian Federation
Doctor of Medical Sciences, Professor, Head of FPK and PPS tuberculosis Control Department,
112, B. Kazachya St., Saratov, 410012
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Review
For citations:
Salina T.Yu., Churkin S.A., Morozova T.I. MOLECULAR GENETIC ANALYSIS AND SPECTRUM OF MUTATIONS IN KATG, INHA, RPOB GENES CODING DRUG RESISTANCE TO ISONIAZID AND RIFAMPICIN IN THOSE SUFFERING FROM TB/HIV CO-INFECTION. Tuberculosis and Lung Diseases. 2016;94(8):54-59. (In Russ.) https://doi.org/10.21292/2075-1230-2016-94-8-54-59
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