MONITORING OF SPECIES DIVERSITY OF NON-TUBERCULOSIS MYCOBACTERIA IN THE SOME RUSSIAN REGIONS USING DNA-STRIPS OF GENOTYPE MYCOBACTERIUM CM/AS (HAIN LIFESCIENCE, GERMANY)
https://doi.org/10.21292/2075-1230-2017-95-5-54-59
Abstract
Goal of study: to define species of non-tuberculosis mycobacteria, isolated from the respiratory tract of the patients from various Russian regions using DNA strips for identification of non-tuberculous mycobacteria.
Materials and methods. 1204 cultures of non-tuberculosis mycobacteria isolated on solid and liquid media from 727 patients in whom tuberculosis/mycobacteriosis was suspected were examined from 2011 to February, 2017. Species of non-tuberculosis mycobacteria were identified using DNA-strips of Hain Lifescience (Germany) GenoType® Mycobacterium CM/AS in compliance with manufacturer's instructions.
Results. 17 species of non-tuberculosis mycobacteria were identified (11 species belonged to the group of those slow-growing and 6 species belonged to fast growing non-tuberculosis mycobacteria). Slow-growing mycobacteria prevailed (564/727, 77.58%) and M. avium was the most prevalent (210/727; 28.89%). The following mycobacteria were found (in descending order): M. gordonae (99/727; 13.62%), M. intracellulare (68/727; 9.35%), M. lentiflavum and M. fortuitum (по 66/727; 9.08%), M. abscessus (49/727; 6.74%), M. kansasii (48/727; 6.60%), M. xenopi (29/727; 3.99%). Prevalence of non-tuberculosis mycobacteria is described in respect of the regions.
About the Authors
T. G. Smirnovа
Central Tuberculosis Research Institute, Moscow
Russian Federation
Candidate of Medical Sciences, Senior Researcher of Microbiological Department
Russian Federation
Candidate of Medical Sciences, Senior Researcher of Microbiological Department
S. N. Аndreevskаya
Central Tuberculosis Research Institute, Moscow
Russian Federation
Candidate of Medical Sciences, Senior Researcher of Microbiological Department
Russian Federation
Candidate of Medical Sciences, Senior Researcher of Microbiological Department
E. E. Lаrionovа
Central Tuberculosis Research Institute, Moscow
Russian Federation
Candidate of Biological Sciences, Senior Researcher of Microbiological Department
Russian Federation
Candidate of Biological Sciences, Senior Researcher of Microbiological Department
I. Yu. Аndrievskаya
Central Tuberculosis Research Institute, Moscow
Russian Federation
Junior Researcher of Microbiological Department
Russian Federation
Junior Researcher of Microbiological Department
V. V. Ustinovа
Central Tuberculosis Research Institute, Moscow
Russian Federation
Junior Researcher of Microbiological Department
Russian Federation
Junior Researcher of Microbiological Department
L. N. Chernousovа
Central Tuberculosis Research Institute, Moscow
Russian Federation
Doctor of Biological Sciences, Professor, Head of Microbiology Department
Russian Federation
Doctor of Biological Sciences, Professor, Head of Microbiology Department
References
1. Guntupova L.D., Borisov S.E., Solovieva I.P. Mycobacterioses in phthisiopulmonary practice: literature review and personal experience. Prakticheskaya Meditsina, 2011, no. 3 (51), pp. 39-50. (In Russ.)
2. Litvinov V.I., Dorozhkova I.R., Makarova M.V., Krasnova M.А., Freyman G.E. Isolation and identification of non-tuberculous mycobacteria. Vestnik RAMN, 2010, no. 3, pp. 7-11. (In Russ.)
3. Otten T.F., Vasiliev А.V. Mikobaketerioz. [Mycobacteriosis]. St. Petersburg, Meditsinskaya Pressa Publ., 2005, 224 p.
4. Edict no. 109 by RF MoH as of 21.03.2003 On Improvement of TB Control Measures in the Russian Federation. Moscow, 2003, 347 p. (In Russ.)
5. Billinger M.E., Olivier K.N., Viboud C., de Oca R.M., Steiner C., Holland S.M., Prevots D.R. Nontuberculous mycobacteria-associated lung disease in hospitalized persons, United States, 1998-2005. Emerg. Infect. Dis., 2009, vol. 15, no. 10, pp. 1562-1569.
6. Blanc P., Dutronc H., Peuchant O., Dauchy F.A., Cazanave C., Neau D., Wirth G., Pellegrin J.L., Morlat P., Mercié P., Tunon-de-Lara J.M., Doutre M.S., Pélissier P., Dupon M. Nontuberculous mycobacterial infections in a French Hospital: A 12-Year Retrospective Study. PLoS One, 2016, vol. 11, no. 12, pp. e0168290.
7. Falkinham J.O. Epidemiology of infection by nontuberculous mycobacteria. Clin. Microbiol. Rev., 1996, vol. 9, no. 2, pp. 177-215.
8. Gitti Z., Neonakis I., Fanti G., Kontos F., Maraki S., Tselentis Y. Use of the GenoType Mycobacterium CM and AS Аssays to analyze 76 nontuberculous mycobacterial isolates from Greece. J. Clin. Microbiol., 2006, vol. 44, no. 6, pp. 2244-2246.
9. Lai C.C., Tan C.K., Chou C.H., Hsu H.L., Liao C.H., Huang Y.T., Yang P.C., Luh K.T., Hsueh P.R. Increasing incidence of nontuberculous mycobacteria, Taiwan, 2000-2008. Emerg. Infect. Dis., 2010, vol. 16, no. 2, pp. 294-296.
10. Lee Y.C., Kim S.B., Gang S.J., Park S.Y., Kim S.R. Acute necrotizing pneumonia combined with parapneumonic effusion caused by Mycobacterium lentiflavum: a case report. BMC Infect Dis., 2015, vol. 15, pp. 354.
11. Mirsaeidi M., Machado R.F., Garcia J.G.N., Schraufnagel D.E. Nontuberculous mycobacterial disease mortality in the United States, 1999-2010: A population-based comparative study. PLoS One, 2014, vol. 9, no. 3, pp. e91879.
12. Molteni C., Gazzola L., Cesari M., Lombardi A., Salerno F., Tortoli E., Lombardi A., Salerno F., Tortoli E., Codecasa L., Penati V., Franzetti F., Gori A. Mycobacterium lentiflavum infection in immunocompetent patient. Emerg. Infect. Dis., 2005, vol. 11, « 1, pp. 119-122.
13. Moore J., Kruijshaar M.E., Ormerod L.P., Drobniewski F., Abubakar I. Increasing number of non-tuberculous mycobacteria in England, Wales and Northern Ireland, 1995-2006. BMC Public Health, 2010, vol. 10, pp. 612.
14. Richter E., Rüsch-Gerdes S., Hillemann D. Evaluation of the GenoType Mycobacterium Assay for identification of mycobacterial species from cultures. J. Clin. Microbiol., 2006, vol. 44, no. 5, pp. 1769-1775.
15. Russo C., Tortoli E., Menichella D. Evaluation of the new GenoType Mycobacterium Assay for identification of mycobacterial species. J. Clin. Microbiol., 2006, vol. 44, no. 2, pp. 334-339.
16. Simons S., van Ingen J., Hsueh P.R., Hung N.V., Dekhuijzen P.N.R., Boeree M.J., van Soolingen D. Nontuberculous mycobacteria in respiratory tract infections, Eastern Asia. Emerg. Infect. Dis., 2011, vol. 17, no. 3, pp. 343-349.
17. Tortoli E. Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin. Microbiol. Rev., 2003, vol. 16, no. 2, pp. 319-354.
18. van der Werf M.J, Ködmön C., Katalinić-Janković V., Kummik T., Soini H., Richter E., Papaventsis D., Tortoli E., Perrin M., van Soolingen D., Žolnir-Dovč M., Thomsen V.Ø. Inventory study of non-tuberculous mycobacteria in the European Union. BMC Infect. Dis., 2014, vol. 14. pp. 62.
Review
For citations:
Smirnovа T.G., Аndreevskаya S.N., Lаrionovа E.E., Аndrievskаya I.Yu., Ustinovа V.V., Chernousovа L.N. MONITORING OF SPECIES DIVERSITY OF NON-TUBERCULOSIS MYCOBACTERIA IN THE SOME RUSSIAN REGIONS USING DNA-STRIPS OF GENOTYPE MYCOBACTERIUM CM/AS (HAIN LIFESCIENCE, GERMANY). Tuberculosis and Lung Diseases. 2017;95(5):54-59. (In Russ.) https://doi.org/10.21292/2075-1230-2017-95-5-54-59
Views:
1540
Email this article 