GENETIC DIVERSITY OF DRUG RESISTANT STRAINS OF MYCOBACTERIUM TUBERCULOSIS IN OMSK REGION
https://doi.org/10.21292/2075-1230-2017-95-7-33-39
Abstract
The article presents the investigation results of the specific epidemic situation on tuberculous infection in Omsk Region in 2006-2015 and molecular genetic features of M. tuberculosis strains with multiple drug resistance circulating in this region. Bacteriological, molecular genetic methods, VNTR-typing were used as well as descriptive techniques of the epidemiological process. Tuberculosis prevalence made 269.2 per 100,000 population. There is an increase in those with bacillary excretion among new cases of respiratory tuberculosis from 39.8% to 53.4%. Drug resistance was detected in 48.0% of new cases. Among drug resistance patterns, MDR made 57%, and extensive drug resistance (XDR) increased from 2.5 to 7.0%. In 2015 prevalence of XDR tuberculosis made 8.9 per 100,000 population in Omsk Region. When performing VNTR-typing of 77 samples of M. tuberculosis DNA with MDR, 27 genetic types were identified. The population of MDR strain of M. tuberculosis is heterogeneous and presented by strains of various genetic families -Beijing, LAM, S,Haarlem,Uganda. The investigation showed that isolates ofBeijing family prevailed (76.6%).
About the Authors
O. A. Pаsechnik
Omsk State Medical University
Russian Federation
Russian Federation
Oksana A. Pasechnik - Candidate of Medical Sciences, Senior Teacher at Epidemiology Department.
9, Mira St., Omsk, 644050 Phone: +7 (3812) 65-06-54.
M. A. Dymovа
Novosibirsk Tuberculosis Research Institute;
Chemical Biology and Fundamental Medicine Institute, Siberian Department of Russian Academy of Sciences
Russian Federation
Russian Federation
Maya A. Dymova - Chemical Biology and Fundamental Medicine Institute, Siberian Department of Russian Academy of Sciences, Candidate of Biological Sciences, Junior Researcher of Pharmacogenomics Laboratory
8, Akad. Lavrentieva Ave., Novosibirsk, 630090 Phone: +7 (3833) 63-51-71.
V. L. Stаsenko
Omsk State Medical University
Russian Federation
Russian Federation
Vladimir L. Stasenko - Doctor of Medical Sciences, Professor, Head of Epidemiology Department.
9, Mira St., Omsk, 644050 Phone: +7 (3812) 65-06-54.
M. P. Tаtаrintsevа
Clinical TB Dispensary
Russian Federation
Russian Federation
Marina P. Tatarintseva - Chief Doctor.
2, Tselinnaya St., Omsk, 644058
I. P. Kolesnikovа
Clinical TB Dispensary
Russian Federation
Russian Federation
Lyubov P. Kolesnikova - Head of Bacteriological Laboratory.
2, Tselinnaya St., Omsk, 644058
E. S. Lyapinа
Clinical TB Dispensary
Russian Federation
Russian Federation
Elena S. Lyapina - Bacteriologist
2, Tselinnaya St., Omsk, 644058
References
1. Dymova M.А., Аlkhovik O.I., Cherednichenko А.G., Khrapov E.А., Petrenko T.I., Filipenko M.L. Genotyping of Mycobacterium tuberculosis isolates with extensive drug resistance. Vestnik Novosibirskogo Gosudarstvennogo Universiteta. Seriya: Biologiya, Klinicheskaya Meditsina, 2013, vol. 11, pp. 110-117. (In Russ.)
2. Kontsevaya I.S., Makurina O.N., Balabanova Ya.M. Study of drug resistance of Mycobacterium tuberculosis in Samara Region. V Mire Nauchnykh Otkrytiy, 2010, no. 4, pp. 51-52. (In Russ.)
3. Mokrousov I.V. Methodical approaches to genotyping to Mycobacterium tuberculosis for evolutional and epidemiological research. Infektsiya and Immunitet, 2012, vol. 2, no. 3, pp. 603-614. (In Russ.)
4. Mokrousov I.V., Narvskaya O.V., Vyazovaya А.А. et al. Gene identification of epidemiologically and clinically significant variant of M. tuberculosis Beijing B0/W148. Tuberculosis and Lung Diseases, 2012, no. 10, pp. 33-36. (In Russ.)
5. Mordyk А.V., Puzyreva L.V., Sitnikova S.V., Ivanova O.G. Tuberculosis with concurrent HIV-infection in Omsk Region from 2008 to 2012. VICH-Infektsiya i Immunosupressii, 2014, vol. 6, no. 2, pp. 106-109. (In Russ.)
6. Narvskaya O.V., Mokrousov I.V., Vyazovaya А.А., Lyalina L.V., Shulgina M.V., Myasnikova E.B. et al. Molecular genetic studies of M. tuberculosis within tuberculosis epidemiological surveillance. Med. Alyans, 2014, no. 1, pp. 75-78. (In Russ.)
7. Pasechnik O.А., Stasenko V.L., Blokh А.I. Epidemiological manifestations of tuberculous infection in the Siberian regions with various prevalence of HIV infection. Epidemiologiya i Vaktsionoprofilaktika, 2015, vol. 14, no. 5 (84), pp. 31-35. (In Russ.)
8. Umpeleva T.V. Molekulyarno-geneticheskaya kharakteristika klinicheskikh izolyatov Mycobacterium tuberculosis, vydelennykh ot bolnykh tuberkulezom v Uralskom federalnom okruge Rossiyskoy Federatsii: Diss. kand. med. nauk. [Molecular genetic characteristics of clinical isolates of Mycobacterium tuberculosis isolated in tuberculosis patients in the Ural Federal District of the Russian Federation. Cand. Diss.]. Yekaterinburg, 2014.
9. Dymova M.A., Cherednichenko A.G., Alkhovik O.I., Petrenko T.I., Filipenko M.L. Characterization of extensively drug-resistant Mycobacterium tuberculosis in Siberia. European Respiratory Journal, 2013, vol. 42, no. 2, pp. 598.
10. Мokrousov I., Vyazovaya A., Otten T., Zhuravlev V., Pavlova E. et al. Mycobacterium tuberculosis Population in Northwestern Russia: An Update from Russian-EU/Latvian Border Region. PLoS ONE 7(7): e41318. doi: 10.1371/journal.pone.0041318.
11. Ribeiro F.K., Pan W., Bertolde A.,Vinhas S.A., Peres R.L., Riley L. Genotypic and spatial analysis of Mycobacterium tuberculosis transmission in a high-incidence urban setting. Clin. Infect. Dis., 2015, vol. 61, no. 5, 758-66. DOI: 10,1093 /CID / civ365.
12. Supply P., Allix C., Lesjean S et al. Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. J. Clin. Microbiol., 2006, vol. 44, no. 12, pp. 4498-4510.
13. Van Embden J.D., Cave M.D., Crawford J.T., Dale J.W., Eisenach K.D., Gicquel B., Herman P., Martin C., McAdam R., Shinnick T.M. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J. Clin. Microbiol., 1993, vol. 31, pp. 406-409.
Review
For citations:
Pаsechnik O.A., Dymovа M.A., Stаsenko V.L., Tаtаrintsevа M.P., Kolesnikovа I.P., Lyapinа E.S. GENETIC DIVERSITY OF DRUG RESISTANT STRAINS OF MYCOBACTERIUM TUBERCULOSIS IN OMSK REGION. Tuberculosis and Lung Diseases. 2017;95(7):33-39. (In Russ.) https://doi.org/10.21292/2075-1230-2017-95-7-33-39
Views:
1178
Email this article 