INVESTIGATION OF SENSITIVITY TO ANTIFUNGAL AGENTS IN ASPERGILLUS FUNGICAUSING BRONCHIAL PULMONARY IN FECTION IN TUBERCULOSIS PATIENTS
https://doi.org/10.21292/2075-1230-2017-95-7-54-60
Abstract
The sensitivity levels of 12 fungal species of Aspergillus, identified when diagnosing pneumonomycosis in tuberculosis patients, to 10 antifungal agents were identified and analyzed. The sensitivity of 202 strains of Aspergillus spp. to antifungal agents was tested by microdilution methods defining minimal inhibitory concentrations in μg/ml (Sensititre system). It was found out that agents causing aspergillosis differed in the level of their resistance to the medication of amphotericin B. Three species with poor sensitivity to this medication were identified: A. terreus (possesses natural resistance to amphotericin B), and A. flavus and A. ochraceus (it is reasonable to test sensitivity to amphotericin B prior to the treatment start). Voriconazolum, intraconazolum and pozaconazolum were found to be active against Aspergillus spp. fungi, but for certain strains of A. ustus (the fungus with poor sensitivity to medications of the azolum group, the sensitivity to be tested prior to the start of treatment) as well as some strains of A. nidulans and A. niger. The article describes a modified method for preparation of Aspergillus spp. spore suspension enhancing safety of sensitivity testing and reliability of the results.
About the Author
A. B. Kulko
Moscow Municipal Scientific Practical Center of Tuberculosis Control, Health Department of Moscow
Russian Federation
Russian Federation
Aleksandr B. Kulko - Candidate of Biological Sciences, Researcher of Department of Laboratory Diagnostics of Tuberculosis and Pathomorphology.
10, Stromynka St., Moscow, 107014 Phone: +7 (499) 268-70-33References
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For citations:
Kulko A.B. INVESTIGATION OF SENSITIVITY TO ANTIFUNGAL AGENTS IN ASPERGILLUS FUNGICAUSING BRONCHIAL PULMONARY IN FECTION IN TUBERCULOSIS PATIENTS. Tuberculosis and Lung Diseases. 2017;95(7):54-60. (In Russ.) https://doi.org/10.21292/2075-1230-2017-95-7-54-60
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