EXPERIENCE OF USING THE MINIMUM INHIBITORY CONCENTRATION METHOD ON LIQUID NUTRITIONAL MEDIA FOR DRUG SUSCEPTIBILITY TESTING OF NON-TUBERCULOUS MYCOBACTERIA
https://doi.org/10.21292/2075-1230-2017-95-8-58-62
Abstract
Goal of the study: analysis of drug resistance of certain species of non-tuberculous mycobacteria through testing by minimum inhibitory concentrations on the fluid nutritional media.
Materials and method: drug resistance of non-tuberculous mycobacteria was tested by the method of minimum inhibitory concentrations on the fluid nutritional media. 51 strains of non-tuberculous mycobacteria from the species of M. intracellulare, M. avium, M. kansasii, M. gordonae, M. fortuitum, M. abscessus were tested. The minimum inhibitory concentration of the drug for slow-growing non-tuberculous mycobacteria was defined using the panel of 13 drugs (SLOWMYCO) and for the fast-growing mycobacteria – the panel of 15 drugs RAPMYCO Sensititre (Trek Diagnostic System, Thermo Scientific, USA) was used. It was proved that the majority of tested fast growing mycobacteria were resistant to ceftriaxone, cefepime, cefoxitin but susceptible to amikacin; and slow growing non-tuberculous mycobacteria were susceptible to clarithromycin, linezolid and amikacin.
About the Authors
O. I. Alkhovik
Novosibirsk Tuberculosis Research Institute
Russian Federation
Russian Federation
Candidate of Medical Sciences, Junior Researcher, Bacteriologist,
81a, Okhotskaya St., Novosibirsk, 630040
T. I. Petrenko
Novosibirsk State Medical University
Russian Federation
Doctor of Medical Sciences, Professor at FPK and PPV Tuberculosis Department
Russian Federation
Doctor of Medical Sciences, Professor at FPK and PPV Tuberculosis Department
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Review
For citations:
Alkhovik O.I., Petrenko T.I. EXPERIENCE OF USING THE MINIMUM INHIBITORY CONCENTRATION METHOD ON LIQUID NUTRITIONAL MEDIA FOR DRUG SUSCEPTIBILITY TESTING OF NON-TUBERCULOUS MYCOBACTERIA. Tuberculosis and Lung Diseases. 2017;95(8):58-62. (In Russ.) https://doi.org/10.21292/2075-1230-2017-95-8-58-62
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