EFFECT OF LASER RADIATION WITH 662 NM WAVE ON THE GROWTH OF MYCOBACTERIUM TUBERCULOSIS IN VITRO
https://doi.org/10.21292/2075-1230-2017-95-8-63-66
Abstract
Goal of the study: to define the effect of various doses of laser radiation with 662 nm wave on the growth of M. tuberculosis in vitro.
Materials and methods. Samples of mycobacterial suspension of M. tuberculosis H37Rv were processed by monopositional light radiation (λ = 662 nm) in six dosing regimens varying in power and duration of the exposure to the light. All samples of mycobacterial suspension of M. tuberculosis were inoculated on the solid nutritional media of Lowenstein-Jensen in triplets for each dose of the exposure to light. Cultures were incubated under 37°С for 90 days with weekly inspection of samples.
Results. Continuous irradiation by diffused laser with 662 nm wave provides the most expressed bacteriostatic and bactericidal effects against M. tuberculosis H37Rv under the density of the energy dose of 234.5 and 703.5 of J/sq.cm. Such a dose was obtained through 5 and 15-minute exposure respectively.
About the Authors
D. A. Bredikhin
Novosibirsk State University
Russian Federation
Engineer of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center
Russian Federation
Engineer of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center
S. D. Nikonov
Novosibirsk Tuberculosis Research Institute;
Novosibirsk State University
Russian Federation
Doctor of Medical Sciences, Professor, Head of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center
Russian Federation
Doctor of Medical Sciences, Professor, Head of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center
A. G. Cherednichenko
Novosibirsk Tuberculosis Research Institute;
Novosibirsk State University
Russian Federation
Senior Engineer of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center
Russian Federation
Senior Engineer of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center
T. I. Petrenko
Novosibirsk Tuberculosis Research Institute;
Novosibirsk State Medical University
Russian Federation
Russian Federation
Novosibirsk Research TB Institute, Doctor of Medical Sciences,
81a, Okhotskaya St., Novosibirsk, 630040
A. V. Ivanenko
Novosibirsk State University
Russian Federation
Candidate of Physico-Mathematical Sciences, Researcher of Laser Physics and Innovative Technologies Department
Russian Federation
Candidate of Physico-Mathematical Sciences, Researcher of Laser Physics and Innovative Technologies Department
M. M. Mirzoev
Novosibirsk State University
Russian Federation
Student of Medicine and Psychology Institute
Russian Federation
Student of Medicine and Psychology Institute
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Review
For citations:
Bredikhin D.A., Nikonov S.D., Cherednichenko A.G., Petrenko T.I., Ivanenko A.V., Mirzoev M.M. EFFECT OF LASER RADIATION WITH 662 NM WAVE ON THE GROWTH OF MYCOBACTERIUM TUBERCULOSIS IN VITRO. Tuberculosis and Lung Diseases. 2017;95(8):63-66. (In Russ.) https://doi.org/10.21292/2075-1230-2017-95-8-63-66
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