ASSESSMENT OF EFFICIENCY OF THE MODIFIED IFA METHOD AIMED TO DETECT ANTIBODIES AGAINST TUBERCULOSIS FOR DEVELOPMENT AND DIAGNOSTICS OF TUBERCULOSIS IN HIV PATIENTS
https://doi.org/10.21292/2075-1230-2018-96-4-20-26
Abstract
The objective of the study: modification of the standard IFA method aimed to detect antibodies against tuberculosis, assessment of its efficiency as a screening tool for prediction and diagnostics of tuberculosis in HIV patients.
Subjects and methods. The modification of the standard IFA method aimed to assess the intensity of humoral anti-tuberculosis immune response included measurement of operational dilution of tested sera in order to enhance its diagnostic value. The modified method was used for the examination in the following groups: 85 active tuberculosis cases, 92 HIV patients with no clinical signs of tuberculosis, and 30 healthy donors.
Results: it is possible to use the modified method in HIV positive people in order to detect those with the highest risk of tuberculosis among them; to provide early diagnostics of active tuberculosis, which is especially crucial at the advanced stages of HIV infection. This method cannot replace the classic methods of tuberculosis diagnostics and it is not recommended for mass screening in HIV negative persons.
About the Authors
A. P. Tsibulkin
Kazan State Medical Academy, Branch of Russian Medical Academy for Professional Development
Russian Federation
Russian Federation
Doctor of Medical Sciences, Professor of Clinical Laboratory Diagnostics Department
Phone: +7 (843) 233-34-72
11, Mushtari St., Kazan, 420012
I. M. Khаertynovа
Kazan State Medical Academy, Branch of Russian Medical Academy for Professional Development
Russian Federation
Russian Federation
Doctor of Medical Sciences, Professor, Head of Infectious Diseases Department
Phone: +7 (987) 2629662; +7 (843) 267-81-17
R. Sh. Vаliev
Kazan State Medical Academy, Branch of Russian Medical Academy for Professional Development
Russian Federation
Russian Federation
Doctor of Medical Sciences, Professor, Head of Phthisiology and Pulmonology Department
Phone: +7 (987) 2961060, +7 (843) 239-96-28
K. S. Khаertynov
Kazan State Medical Academy, Branch of Russian Medical Academy for Professional Development
Russian Federation
Russian Federation
Candidate of Biological Sciences, Head of Central Research Laboratory
Phone: +7(987) 407 87 70; +7 (843) 203-60-52
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Review
For citations:
Tsibulkin A.P., Khаertynovа I.M., Vаliev R.Sh., Khаertynov K.S. ASSESSMENT OF EFFICIENCY OF THE MODIFIED IFA METHOD AIMED TO DETECT ANTIBODIES AGAINST TUBERCULOSIS FOR DEVELOPMENT AND DIAGNOSTICS OF TUBERCULOSIS IN HIV PATIENTS. Tuberculosis and Lung Diseases. 2018;96(4):20-26. (In Russ.) https://doi.org/10.21292/2075-1230-2018-96-4-20-26
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