The predictive function of rates of matrix metalloproteinases/inhibitors system when assessing reparative changes in the lung tissue in those with infiltrate pulmonary tuberculosis

The objective: to study the rates of matrix metalloproteinases/inhibitors system in infiltrate pulmonary tuberculosis during changes occurring in the intensive phase of treatment in order to develop a statistic model aimed to predict reparative changes.

Subjects and methods. The test of ELISA (Bender MedSystems, USA) was used to measure the levels of MMP-1, MMP-8,-9. TIMM and α₂-macroglobulin in blood serum of 35 patients with verified infiltrate pulmonary tuberculosis and various reparative changes during the intensive phase of treatment. The extension of infiltrative changes in the lung tissue was calculated (Vitrea, Nodule Analysis). The software of Statistica 10, ANOVA Repeated Measures and multiple discriminant analysis were used.

Results. Infiltrate pulmonary tuberculosis disrupts the balance in MMP/inhibitors system regardless of the character of reparative changes: MMP-9 goes higher and the reference level of TIMM-1 and α₂-macroglobulin persists. The level of MMP-9 significantly goes down but does not become normal in all variants of the disease involution. Persisting high levels of MMP-8 reduces the chances of cavity healing, while the increasing level of MMP-1 promotes resolution of cavities. To predict the character of reparative changes upon the completion of the intensive phase of treatment of infiltrate pulmonary tuberculosis, the combination of levels of MMP/inhibitors with lung tissue changes during the intensive phase of treatment is the most informative.

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