In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue
https://doi.org/10.21292/2075-1230-2019-97-7-28-33
Abstract
The objective: to investigate the anti-tuberculosis effect of laser photodynamic inactivation (PDI) of M. tuberculosis H37Rv in vitro by methylene blue (MB) in the minimum concentration (1 μg/ml) with laser radiation of 662 nm.
Subjects and methods. A comparative analysis of the intensity of growth of Mycobacterium tuberculosis H37Rv after laser irradiation and laser FDI by MB with different doses of light energy was carried out.
Results. Laser radiation with a wavelength of 662 nm was found to have an inhibitory effect on the growth of M. tuberculosis H37Rv. FDI of Mycobacterium tuberculosis was first registered in the presence of a minimum concentration of MB (1 µg/ml) which suppressed colony growth by 97 and 93% when they were processed by radiation with a wavelength of 662 nm with the lowest density of doses of light energy (46.9 and 93.75 J/cm2).
About the Authors
D. A. Bredikhin
Novosibirsk State University; Novosibirsk State Regional Clinical Tuberculosis Hospital
Russian Federation
Russian Federation
Demid A. Bredikhin - Leading Engineer of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center.
14, Vavilova St., Novosibirsk, 630082
S. D. Nikonov
Novosibirsk Tuberculosis Research Institute; Novosibirsk State University; Novosibirsk State Regional Clinical Tuberculosis Hospital
Russian Federation
Russian Federation
Sergey D. Nikonov - Doctor of Medical Sciences, Professor, Head of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center.
14, Vavilova St., Novosibirsk, 630082, Phone: +7 (383) 225-59-81
A. G. Cherednichenko
Novosibirsk Tuberculosis Research Institute; Novosibirsk State University
Russian Federation
Russian Federation
Andrey G. Cherednichenko - Leading Engineer of Laboratory for Use of Quantum Materials, Devices and Systems within Cross-Disciplinary Quantum Center.
14, Vavilova St., Novosibirsk, 630082, Phone: +7 (383) 203-83-62
T. I. Petrenko
Novosibirsk Tuberculosis Research Institute
Russian Federation
Russian Federation
Tatiana I. Petrenko - Doctor of Medical Sciences, Chief Researcher.
81a, Okhotskaya St., Novosibirsk, 630040, Phone: +7 (383) 203-83-58A. I. Korbut
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center of Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Anton I. Korbut - Junior Researcher of Endocrinology Laboratory.
2, Timakova St., Novosibirsk, 630117, Phone: +7 (383) 336-40-22References
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Review
For citations:
Bredikhin D.A., Nikonov S.D., Cherednichenko A.G., Petrenko T.I., Korbut A.I. In vitro photodynamic inactivation of Mycobacterium tuberculosis by methylene blue. Tuberculosis and Lung Diseases. 2019;97(7):28-33. (In Russ.) https://doi.org/10.21292/2075-1230-2019-97-7-28-33
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