Неэффективность вакцины BCG для защиты от туберкулезной инфекции у мышей линии B10.M (H2f) и иммунный ответ на антигены микобактерий
https://doi.org/10.21292/2075-1230-2019-97-7-48-55
Abstract
Objective: to identify specific features of the immune response making BCG vaccine ineffective in mice carrying H2f allele of the main complex of tissue compatibility.
Subjects and methods. Inbred lines of B10.M (H2f) and B10 (H2b) mice vaccinated and not vaccinated with BCG and infected with M. tuberculosis H37Rv, were compared in terms of survival after the infection, the number of mycobacteria in the lungs, the ability of T-lymphocytes to recognize mycobacterial antigens and produce interferon-γ (IFN-γ ) in response to mycobacterial antigens and non-specific stimulation of T-receptors.
Results. It was found out that B10.M mice were unable to produce T-cells by the lymphoid organs (spleen) and lungs to produce IFN-γ in response to long-term stimulation of mycobacterial antigens in chronic infection, although the recognition of these antigens, as well as the ability to produce IFN-γ in response to non-specific binding of T-receptors with anti-CD3 antibodies, were completely preserved. It was demonstrated that the defect in IFN-γ production manifested at a late stage of infection regardless of prior BCG vaccination, and hypothesized that it was rather associated with the phenomenon of specific "immunological depletion" of T-cells in mice carrying some allelic variants of H2 complex.About the Authors
M. V. Korotetskаya
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Maria V. Korotetskaya - Acting Senior Researcher of Immunogenetics Laboratory.
2, Yauzskaya Alleya, Moscow, 107564, Phone: +7 (499) 785-90-72
P. G. Bаykuzinа
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Polina G. Baykuzina - Student of the 5th Year at the Biomedical Engineering and Informatics Department, Lomonosov Moscow State University.
2, Yauzskaya Alleya, Moscow, 107564, Phone: +7 (499) 785-90-72
A. S. Аpt
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Aleksandr S. Apt - Head of Immunogenetics Laboratory.
2, Yauzskaya Alleya, Moscow, 107564, Phone: +7 (499) 785-90-72
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For citations:
Korotetskаya M.V., Bаykuzinа P.G., Аpt A.S. Неэффективность вакцины BCG для защиты от туберкулезной инфекции у мышей линии B10.M (H2f) и иммунный ответ на антигены микобактерий. Tuberculosis and Lung Diseases. 2019;97(7):48-55. (In Russ.) https://doi.org/10.21292/2075-1230-2019-97-7-48-55
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