The effect of real-time PCR as a part of diagnostic procedure on the efficacy of treatment of multiple drug resistant tuberculosis
https://doi.org/10.21292/2075-1230-2019-97-9-40-44
Abstract
The objective of the study: to investigate the effect of the molecular genetic test of PCR-RT within the procedure of microbiological diagnosis of tuberculosis on the efficacy of chemotherapy in patients with multiple drug resistant tuberculosis in the Mary-El Republic.
Subjects and methods. 344 patients with multiple drug resistant tuberculosis were enrolled in the study. The efficacy of chemotherapy was assessed based on sputum conversion confirmed by culture.
Results. It was demonstrated that PCR-RT for determining mutations in the diagnostic material samples associated with drug resistance to rifampicin, isoniazid, and fluoroquinolones, and the early prescription of an adequate chemotherapy regimen based on this study, followed by amendment of the treatment regimen according to the results of phenotypic drug susceptibility tests, significantly (on average from 3.6 to 2.5 months, p <0.05) reduced the time required for sputum conversion confirmed by culture, which reduced the time of in-patient treatment.
About the Authors
L. V. Petrova
Republican TB Dispensary
Russian Federation
Head of Bacteriological Laboratory
Russian Federation
Head of Bacteriological Laboratory
E. V. Sevastyanova
Central Tuberculosis Research Institute
Russian Federation
Doctor of Biological Sciences, Leading Researcher of Microbiology Department
Russian Federation
Doctor of Biological Sciences, Leading Researcher of Microbiology Department
A. M. Vasilieva
Republican TB Dispensary
Russian Federation
Deputy Head Physician on Reporting and Statistics
Russian Federation
Deputy Head Physician on Reporting and Statistics
E. A. Kuklina
Republican TB Dispensary
Russian Federation
Phthisiologist
Russian Federation
Phthisiologist
Yu. A. Soloviev
Republican TB Dispensary
Russian Federation
Head Physician
Russian Federation
Head Physician
L. N. Chernousova
Central Tuberculosis Research Institute
Russian Federation
Doctor of Biological Sciences, Professor, Head of Microbiological Department
Russian Federation
Doctor of Biological Sciences, Professor, Head of Microbiological Department
References
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Review
For citations:
Petrova L.V., Sevastyanova E.V., Vasilieva A.M., Kuklina E.A., Soloviev Yu.A., Chernousova L.N. The effect of real-time PCR as a part of diagnostic procedure on the efficacy of treatment of multiple drug resistant tuberculosis. Tuberculosis and Lung Diseases. 2019;97(9):40-44. (In Russ.) https://doi.org/10.21292/2075-1230-2019-97-9-40-44
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