Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection
https://doi.org/10.21292/2075-1230-2020-98-4-48-52
Abstract
In addition to standard skin tests (Mantoux test with 2 TU PPD-L and diaskintest) for the diagnosis of tuberculosis infection, in vitro tests are used. One of these tests is T-SPOT.TB being more widely used in recent years.
The objective: to evaluate the effectiveness of T-SPOT.TB test for early detection of tuberculosis infection in children and adolescents in Samara Region.
Subjects and methods. From 2016 to 2019, results of T-SPOT.TB tests performed in 596 children aged 2 to 17 years inclusive were analyzed; those children had no immunodiagnosis of tuberculosis infection using skin tests since their parents refused to have it.
Results. It was found out that the major reason for refusing skin tests was the “fear” of visiting a TB dispensary if the result had been positive — 38.43% (n = 229). The latent tuberculosis infection according to the results of T-SPOT.TB among children with concomitant pathology made 2.6%, among healthy children – 0.7%.
Conclusion. T-SPOT.TB test may be used as an alternative method for diagnosis of tuberculosis infection, should the parent refuse to have skin tests. In children with concomitant pathology, T-SPOT.TB test can serve as a leading method for immunodiagnosis of tuberculosis.
The authors state that they have no conflict of interests.
About the Authors
E. P. Eremenko
Samara State Medical University
Russian Federation
Ekaterina P. Eremenko Candidate of Medical Sciences, Assistant of Phthisiology and Pulmonology Department.
Russian Federation
Ekaterina P. Eremenko Candidate of Medical Sciences, Assistant of Phthisiology and Pulmonology Department.
E. A. Borodulina
Samara State Medical University
Russian Federation
Elena A. Borodulina Doctor of Medical Sciences, Professor, Head of Phthisiology and Pulmonology Department.
Russian Federation
Elena A. Borodulina Doctor of Medical Sciences, Professor, Head of Phthisiology and Pulmonology Department.
I. A. Sergeeva
Mother and Child, Pediatric Polyclinic, IDK Private Medical Center
Russian Federation
Russian Federation
Irina A. Sergeeva Head Physician.
Phone: +7 (846) 342-62-62.
D. A. Kudlay
Immunology Research Institute by the Federal Medical Biological Agency
Russian Federation
Dmitry A. Kudlay Doctor of Medical Sciences, Leading Researcher of Personalized Medicine and Molecular Immunology Laboratory.
Russian Federation
Dmitry A. Kudlay Doctor of Medical Sciences, Leading Researcher of Personalized Medicine and Molecular Immunology Laboratory.
B. E. Borodulin
Samara State Medical University
Russian Federation
Boris E. Borodulin Doctor of Medical Sciences, Professor, Professor of Phthisiology and Pulmonology Department.
Russian Federation
Boris E. Borodulin Doctor of Medical Sciences, Professor, Professor of Phthisiology and Pulmonology Department.
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Review
For citations:
Eremenko E.P., Borodulina E.A., Sergeeva I.A., Kudlay D.A., Borodulin B.E. Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection. Tuberculosis and Lung Diseases. 2020;98(4):48-52. (In Russ.) https://doi.org/10.21292/2075-1230-2020-98-4-48-52
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