Effect of the combination of theophylline and budesonide on production of proinflammatory cytokines by blood cells of patients with chronic obstructive pulmonary disease
https://doi.org/10.21292/2075-1230-2021-99-10-14-22
Abstract
The objective: to evaluate the ability of the combination of theophylline and budesonide to suppress proinflammatory cytokine production byblood cells in patients with chronic obstructive pulmonary disease (COPD).
Subjects and Methods. Peripheral blood mononuclear cells (PBMCs) or whole blood cells of COPD patients (n = 27) were incubated with budesonide (10 nM), theophylline (1 μM), or the combination thereof and stimulated with phytohemagglutinin (PHA) or phorbol myristate acetate (PMA) and ionomycin. The enzyme immunoassay was used to evaluate the secretion of thymic stromal lymphopoietin (TSLP), macrophage migration inhibitory factor (MIF), interleukin 17A (IL-17A), IL-33, and other mediators of PBMC cells, and induced PHA. The flow cytometry was used to analyze intracellular production of proinflammatory cytokines stimulated by PMA/ionomycin in T-helpers (CD4+) and cytotoxic T-lymphocytes (CD8+).
Results. Theophylline reduced the secretion of IL-4 and IL-17A by PBMC cells. The combination of budesonide with theophylline inhibited the synthesis of IL-4, IL-5, IL-8, IL-13, IL-17A, IL-33, TSLP, MIF by PBMC cells as well as the production of IL-4, IL-8, tumor necrosis factor-α, and interferon-γ by cytotoxic T-lymphocytes and T-helpers. The combination of theophylline and budesonide had a more pronounced inhibitory effect on the production of IL-4 and IL-8 by PBMC cells as well as the synthesis of IL-4 by CD4+ T-cells and IL8 by CD8+ T-lymphocytes versus the effect of monotherapy with budesonide.
About the Authors
A. G. Kadushkin
Belorussian State Medical University
Belarus
Belarus
Aleksey G. Kadushkin, Candidate of Medical Sciences, Associate Professor of Biological Chemistry Department
83, Dzerzhinskogo Ave., Minsk, 220116, phone: (37517) 373-93-92
A. D. Taganovich
Belorussian State Medical University
Belarus
Belarus
Anatoliy D. Taganovich, Doctor of Medical Sciences, Professor, Head of of Biological Chemistry Department
83, Dzerzhinskogo Ave., Minsk, 220116, phone: (37517) 277-17-64
L. V. Movchan
Republican Scientific Practical Center of Children’s Oncology, Hematology and Immunology
Belarus
Belarus
Lyudmila V. Movchan, Candidate of Biological Sciences, Laboratory Diagnostics Physician of Clinical Diagnostic Laboratory
43, Frunzenskaya St., Borovlyany, Minsky District, Minsky Region, 223053, phone: (37517) 265-40-89
E. I. Talabaeva
Minsk Clinical Consulting and Diagnostic Center
Belarus
Belarus
Elina I. Talabaeva, Pulmonologist of Consulting Department
10, Semashko St., Minsk, Belarus, 220045, phone: (37517) 277-19-87
A. V. Plastinina
Minsk Clinical Consulting and Diagnostic Center
Belarus
Belarus
Alena V. Plastinina, Pulmonologist of Consulting Department
10, Semashko St., Minsk, Belarus, 220045, phone: (37517) 277-19-87
T. V. Shman
Republican Scientific Practical Center of Children’s Oncology, Hematology and Immunology
Belarus
Belarus
Tatiana V. Shman, Candidate of Biological Sciences, Head of Immunological Laboratory
43, Frunzenskaya St., Borovlyany, Minsky District, Minsky Region, 223053, phone: (37517) 265-40-89
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Review
For citations:
Kadushkin A.G., Taganovich A.D., Movchan L.V., Talabaeva E.I., Plastinina A.V., Shman T.V. Effect of the combination of theophylline and budesonide on production of proinflammatory cytokines by blood cells of patients with chronic obstructive pulmonary disease. Tuberculosis and Lung Diseases. 2021;99(10):14-22. (In Russ.) https://doi.org/10.21292/2075-1230-2021-99-10-14-22
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