The new attenuated strain of Mycobacterium tuberculosis BN. Characteristics and vaccine properties
https://doi.org/10.21292/2075-1230-2021-99-10-60-65
Abstract
The objective of the study: to obtain a live attenuated strain and investigate its properties by multiple cultures of the virulent strain of Mycobacterium tuberculosis H37Rv.
Subjects and Methods. The original virulent strain H37Rv was subcultured 70 times in 7H9 liquid medium. Genetic properties of the new strain, degree of avirulence, and vaccine properties were studied.
Results. Mycobacteria of the new strain MtbBN lost their virulence to inbred mice. Eight mutations were identified by whole genome sequencing: single nucleotide insertions and deletions (in/del) distinguishing the MtbBN and H37Rv strains. The MtbBN strain demonstrated vaccine potential at the BCG level. Additionally, in some genetic models, the attenuated strain was highly effective in protecting inbred mice when infected with Mtb H37Rv as opposed to BCG.
About the Authors
B. V. Nikonenko
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Boris V. Nikonenko, Doctor of Medical Sciences, Senior Researcher of Immunology Department
2, Yauzskaya Alleya, Moscow, 107564
T. L. Аzhikina
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
Tatyana L. Аzhikina, Doctor of Biological Sciences, Head of Laboratory of Regulatory Transcriptomics
6/ 10, Miklukho-Maklaya St., Moscow, 117871
A. S. Grigorov
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
Artem S. Grigorov, Researcher of Laboratory of Regulatory Transcriptomics
6/ 10, Miklukho-Maklaya St., Moscow, 117871
I. A. Linge
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
Irina A. Linge, Candidate of Biological Sciences, Senior Researcher of Immunology Department
6/ 10, Miklukho-Maklaya St., Moscow, 117871
N. N. Logunova
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Nadezhda N. Logunova, Candidate of Biological Sciences, Senior Researcher of Immunology Department
2, Yauzskaya Alleya, Moscow, 107564
S. I. Kayukova
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Sveltana I. Kayukova, Doctor of Medical Sciences, Leading Researcher, Head of Microbiome Laboratory of Immunology Department
2, Yauzskaya Alleya, Moscow, 107564
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Review
For citations:
Nikonenko B.V., Аzhikina T.L., Grigorov A.S., Linge I.A., Logunova N.N., Kayukova S.I. The new attenuated strain of Mycobacterium tuberculosis BN. Characteristics and vaccine properties. Tuberculosis and Lung Diseases. 2021;99(10):60-65. (In Russ.) https://doi.org/10.21292/2075-1230-2021-99-10-60-65
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