Expression of the TSC1 and TSC2 Genes in Peripheral Blood Leukocytes of Patients with Chronic Pulmonary Sarcoidosis
https://doi.org/10.58838/2075-1230-2025-103-5-52-56
Abstract
The objective: to study the level of mRNA expression of the TSC1 and TSC2 genes in peripheral blood leukocytes of patients with chronic pulmonary sarcoidosis and healthy individuals.
Subjects and Methods. A total of 58 people were examined, of them 28 people were patients with chronic pulmonary sarcoidosis (stage II of the disease) without treatment, their average age made 42.11±2.21 years, and 30 people were included to Comparison Group (healthy individuals) with the average age of 43.03±1.84 years. Real-time PCR was used to assess the level of mRNA expression of the TSC1 and TSC2 genes in peripheral blood leukocytes (PBL) of patients with pulmonary sarcoidosis and healthy individuals.
Results. In the group of patients with sarcoidosis (stage II of the disease), testing peripheral blood leukocytes revealed a significant decrease in expression of mRNA of the TSC1 gene (p=0.007) and a significant increase in the number of transcripts of the TSC1 gene (p=0.0002) compared to healthy individuals matched for age.
Keywords
pulmonary sarcoidosis,
chronic course of the disease,
expression,
mRNA of the gene TSC1,
mRNA of the gene TSC2
Supporting agencies: This research was funded by the federal budget for implementation of the state task of Karelian Research Center of the Russian Academy of Science (Topic: FMEN-2022-0009).
About the Authors
I. E. Malysheva
Karelian Research Center, Russian Academy of Sciences
Russian Federation
Russian Federation
Irina E. Malysheva - Candidate of Biological Sciences, Senior Researcher of Genetics Laboratory, Institute of Biology, Karelian Research Center, Russian Academy of Sciences.
11 Pushkinskaya St., Petrozavodsk, 185910
Phone: +7 (8142) 76-60-40
O. V. Balan
Karelian Research Center, Russian Academy of Sciences
Russian Federation
Russian Federation
Olga V. Balan - Candidate of Biological Sciences, Senior Researcher of Genetics Laboratory, Institute of Biology, Karelian Research Center, Russian Academy of Sciences.
11 Pushkinskaya St., Petrozavodsk, 185910
Phone: +7 (8142) 76-60-40
E. L. Tikhonovich
V.A. Baranov Republican Hospital
Russian Federation
Russian Federation
Ella L. Tikhonovich - Head of Respiratory Therapy Department.
3 Pirogova St., Petrozavodsk, 185002
Phone: +7 (8142) 76-39-10
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Review
For citations:
Malysheva I.E., Balan O.V., Tikhonovich E.L. Expression of the TSC1 and TSC2 Genes in Peripheral Blood Leukocytes of Patients with Chronic Pulmonary Sarcoidosis. Tuberculosis and Lung Diseases. 2025;103(5):52-56. (In Russ.) https://doi.org/10.58838/2075-1230-2025-103-5-52-56
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