ANALYSIS OF GENE EXPRESSION IN THE LUNG TISSUE IN MICE CONGENIC AS PER H2-LINE COMPLEX WITH VARIOUS SEVERITY OF TUBERCULOUS INFECTION COURSE

Abstract

With the help of DNA-chip on the Illumina platform the comparative analysis was performed of all genes expression in the lung tissue of two congenic lines of mice – B6 resistant to tuberculosis and much more sensitive B6.1-9.5.7, diŠerent from B6 only with small region of the 17th chromosome carrying MNC genes of class II. The comparison included healthy mice and mice infected by air-borne transmission~500 CFUЕ of Mycobacterium tuberculosis H37Rv three weeks prior to mRNA isolation. The results were verified by quantitative PCR in real time and it showed that the part of genome which was diŠerent in the mice lines was so polymorphous that technically it was impossible to use it to compare expression of genes contained in it by methods based on DNA-chips developed specifically for B6 line. On the contrary the eŠect of the infection on expression of all other genes was defined with high accuracy. It has been shown that in mice of resistant B6 line the number of genes changing the expression at the early phase of the infection is significantly higher compared to the susceptible line of B6.I-9.5.7. The main diŠerence between two lines of mice is the expression of genes responsible for immune response regulation of cellular cycle and liposome activity. One can suppose that the resistance is based on the involvement into interaction with the causative agent of the significant number of metabolic ways and their regulators.