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STUDY OF NOSOCOMIAL INFECTION BY IS6110 RFLP AND MIRU-VNTR METHODS IN THE UNIT FOR PATIENTS WITH MULTIDRUG-RESISTANT TUBERCULOSIS

https://doi.org/10.21292/2075-1230-2014-0-1-43-46

Abstract

Drug susceptibility tests were monthly carried out in 41 patients treated in the multidrug-resistant tuberculosis unit. All cultures were examined using the molecular genetic methods IS6110 RFLP and MIRU-VNTR in 10 patients with a changed result of the drug susceptibility test. Molecular genetic examination of nosocomial infection showed that the likelihood of in-hospital infection in the specialized hospital unit for patients with multidrug-resistant tuberculosis was 19.5%.

About the Authors

N. A. Vlasova
Архангельский клинический противотуберкулезный диспансер
Russian Federation


V. N. Stepanshina
ГНЦ прикладной микробиологии и биотехнологии
Russian Federation
г. Оболенск


T. N. Mukhina
ГНЦ прикладной микробиологии и биотехнологии
Russian Federation
г. Оболенск


R. I. Mironova
ГНЦ прикладной микробиологии и биотехнологии
Russian Federation
г. Оболенск


A. O. Maryandyshev
Северный государственный медицинский университет
Russian Federation
г. Архангельск


References

1. Баранов А. А., Марьяндышев А. О. Применение методов молекулярной биологии для исследования микобактерий туберкулеза // Пробл. туб. – 2008. – № 4. – С. 3-8.

2. Горина Г. П., Ажикина Т. Л., Тарасова И. В. и др. Нозокомиальная инфекция среди больных туберкулезом легких с множественной лекарственной устойчивостью в Архангельской области // Туб. – 2012. – № 6. – С. 26.

3. Мясникова Е. Б. Клинико-эпидемиологические критерии диагностики нозокомиальной туберкулезной инфекции у пациентов противотуберкулезных учреждений. Материалы 1-го конгресса национальной ассоциации фтизиатров. – 2012. – С. 389-391.

4. Приказ Министерства здравоохранения РФ № 109 от 23 марта 2003 г.

5. Профилактика туберкулеза в лечебно-профилактических учреждениях при дефиците необходимых ресурсов. Методические рекомендации. – ВОЗ, 1999.

6. Antonio Rivero, Manuel Marques, Jesus Santos et al. High rate of tuberculosis reinfection during a nosocomial outbreak of multidrug-resistant tuberculosis caused by Mycobacterium bovis strain B // Clin. Infect. Deseas. – 2001. – Vol. 32. – P.159-161.

7. van Embden J. D., Cave M. D., Crawford J. T. et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology // J. Clin. Microbiol. – 1993. – Vol. 31. – P. 406-409.

8. Janet C. Mohle-Boetani, Vanessa Miguelino. Daniel H. et al. Tuberculosis outbreak in a housing unite for human immunodeficiency virus-infected patients in a correctional facility: transmission risk factors and effective outbreak control // Clin. Infect. Dis. – 2002. – Vol. 34, № 5. – Р. 668-676. doc: 10.1086/338815.

9. Luu Thi Lien, Nguyen Thi Le Hang, Nabuyki Kobayashi et al. Prevalence and risk factor for tuberculosis infection among hospital workers in Hanoi.Vietnam.Plosone4(8);e6798doi,10,1371/joumal.pone.0006798.

10. Nguyen D., Brassard P., Menzies D. et al. Genomic characterization of an endemic Mycobacterium tuberculisis strain: evolutionary and epidemiologic implications// J. Clin. Microbiol. – 2004. – Vol. 42. – P. 2573-2580.

11. Rick Scott Closes A.G. Holley State Hospit. Despite largest tuberculosis outbreak in country huffpost Miami www.huffingtonpost.cjm/2012/07/09/florida-tuberculosis-outbreak-keptsecret_n_1658916.htmi

12. Sanjay Basu M. S., Jason R. Andrews MD., Eric M. Poolman MD. еt al. Prevention of nosocomial transmission of extensively drug-resistant tuberculosis in rural South African district hospitals; an epidemiological modeling study // Lancet. – 2007. – Vol. 370, issue 9597. – P. 1500-1507.

13. Supply Ph. et al. Proposal for standardization of optimized mycobacterial interspersed repetitive unit–variable-number tandem repeat typing of Mycobacterium tuberculosis // J. Clin. Microbiol. – 2006. – Vol. 44. – P. 4498-4510. ДЛЯ КОРРЕСПОНДЕНЦИИ:


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For citations:


Vlasova N.A., Stepanshina V.N., Mukhina T.N., Mironova R.I., Maryandyshev A.O. STUDY OF NOSOCOMIAL INFECTION BY IS6110 RFLP AND MIRU-VNTR METHODS IN THE UNIT FOR PATIENTS WITH MULTIDRUG-RESISTANT TUBERCULOSIS. Tuberculosis and Lung Diseases. 2014;(1):43-46. (In Russ.) https://doi.org/10.21292/2075-1230-2014-0-1-43-46

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ISSN 2075-1230 (Print)
ISSN 2542-1506 (Online)