Comparative Evaluation of Effectiveness of Reagent Kits Amplitub-NTMB Differentsiatsia and MALDI-TOF for Mass Spectrometry for Identification of Non-Tuberculous Mycobacteria Species
https://doi.org/10.58838/2075-1230-2026-104-1-38-43
Abstract
The objective: to compare results of species identification of non-tuberculous mycobacteria by PCR (Amplitub-NTM Differentsiatsia) and MALDI-TOF mass spectrometry in clinical samples and mycobacterial cultures.
Subjects and Methods. 40 clinical samples and 110 cultures of non-tuberculous mycobacteria were tested. Two methods were used for testing: Amplitub-NTM Differentsiatsia and MALDI-TOF mass spectrometry.
Results. A high level of agreement between results of MALDI-TOF mass spectrometry and Amplitub-NTMB Differentsiatsia PCR kit was demonstrated. PCR confirmed its high efficiency, detecting non-tuberculous mycobacteria in 89.3% of samples; the final match of species identification with MALDI-TOF in both clinical samples and cultures made 85.1%. Both methods demonstrate a high level of agreement and they complement each other. The PCR kit can be used as the main screening method, especially in TB laboratories. If negative or indeterminate results are obtained, combination with MALDI-TOF or sequencing is recommended The optimal diagnostic strategy involves the use of PCR as a part of comprehensive approach to non-tuberculous mycobacteria identification.
About the Authors
P. I. Eliseev
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Platon I. Eliseev, Candidate of Medical Sciences, Head of Microbiology Research Laboratory
Build. 2, 4 Dostoevskiy St., Moscow, 127473;
Phone: +7 (495) 631-15-15
A. A. Kazyulina
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Anastasia A. Kazyulina, Head of Research Laboratory of Molecular Biotechnology
Build. 2, 4 Dostoevskiy St., Moscow, 127473;
Phone: +7 (495) 631-15-15
G. V. Pay
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Galina V. Pay, Candidate of Medical Sciences, Clinical Laboratory Diagnostics Physician of Laboratory Diagnostics Department
Build. 2, 4 Dostoevskiy St., Moscow, 127473;
Phone: +7 (495) 631-15-15
E. Yu. Gosteva
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Ekaterina Yu. Gosteva, Junior Researcher of Microbiology Research Laboratory
Build. 2, 4 Dostoevskiy St., Moscow, 127473;
Phone: +7 (495) 631-15-15
N. N. Gorshkova
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Natalia N. Gorshkova, Junior Researcher of Microbiology Research Laboratory
Build. 2, 4 Dostoevskiy St., Moscow, 127473;
Phone: +7 (495) 631-15-15
E. O. Kuznetsov
National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of Health
Russian Federation
Russian Federation
Evgeniy O. Kuznetsov, Head of Laboratory Diagnostics Department
Build. 2, 4 Dostoevskiy St., Moscow, 127473;
Phone: +7 (495) 631-15-15
References
1. Veselova E.I., Kuznetsova E.N., Peregudova A.B. et al. Generalized mycobacteriosis in HIV patients. Tuberculosis and Lung Diseases, 2024, vol. 102, no. 5, pp. 50-57. (In Russ.) https://doi.org/10.58838/2075-1230-2024-102-5-50-57
2. Kazyulina A.A., Panova A.E., Vinokurov A.S., Baybekov K.S., Bayrakova A.L., Samoylova A.G., Vasilyeva I.A. Species identification of nontuberculous mycobacteria using MALDI-TOF mass spectrometry and full-genome NGS sequencing: a comparative analysis. Epidemiologiya i Infektsionnye Bolezni, 2024, vol. 29, no. 4, pp. 217-221. (In Russ.)
3. Lyamin A.V., Chernousova L.N., Morozova T.I. et al. Non-tuberculous mycobacteria: modern possibilities of species identification. Clinical Microbiology and Antimicrobial Chemotherapy, 2017, vol. 19, no. 3, pp. 186-197. (In Russ.)
4. Makaryants N.N., Zaytseva A.S. Netuberkuleznyye mikobakteriozy. Problemy lecheniya i nablyudeniya patsiyentov. [Non-tuberculous mycobacteriosis. Treatment and follow-up problems]. Moscow, FGBNU TSNIIT Publ., 2020, 128 p.
5. A set of reagents for diagnosis of mycobacteria Amplitub-NTMB-Differentsiatsia by LLC Synthol. (In Russ.) Available: http://www.syntol.ru/catalog/dlya-diagnostiki-tuberkulyeza/ Accessed August 11, 2025
6. Smirnova T.G., Andreevskaya S.N., Ustinova V.V. et al. Discriminatory power of multiplex PCR for detection of mycobacterial co-infection. Bulletin of RSMU, 2024, no. 4, pp. 45-54. (In Russ.)
7. Umpeleva T.V., Shulgina M.V., Vakhrusheva D.V., Eremeeva N.I. Antimicrobial susceptibility of Mycobacterium avium complex mycobacteria isolated from patients in Ural Federal District of the Russian Federation. Clinical Microbiology and Antimicrobial Chemotherapy, 2022, vol. 24, no. 2, pp. 147-154. (In Russ.)
8. Ustinova V.V. Sovershenstvovaniye molekulyarno-geneticheskikh metodov vyyavleniya netuberkuleznykh mikobakteriy i mikobakteriy tuberkuloznogo kompleksa: Avtoref. dis. kand. med. nauk. [Improving molecular genetic methods for detecting non-tuberculous mycobacteria and mycobacteria of the tuberculosis complex: Synopsis of Cand. Diss.]. Moscow, 2023, 26 p.
9. Daley C.L., Iaccarino J.M., Lange C. et al. Treatment of nontuberculous mycobacterial pulmonary disease: an official ATS/ERS/ESCMID/IDSA clinical practice guideline. Clinical Infection Diseases, 2020, vol. 71, no. 4, pp. 905-913. https://doi.org/10.1093/cid/ciaa1125
10. Eremeeva N., Belousova K., Umpeleva T. et al. Identification of nontuberculous mycobacteria (NTM) isolated from patients from the TB hospital. European Respiratory Journal, 2019, vol. 54, no. 10, pp. PA4643.
Review
For citations:
Eliseev P.I., Kazyulina A.A., Pay G.V., Gosteva E.Yu., Gorshkova N.N., Kuznetsov E.O. Comparative Evaluation of Effectiveness of Reagent Kits Amplitub-NTMB Differentsiatsia and MALDI-TOF for Mass Spectrometry for Identification of Non-Tuberculous Mycobacteria Species. Tuberculosis and Lung Diseases. 2026;104(1):38-43. (In Russ.) https://doi.org/10.58838/2075-1230-2026-104-1-38-43
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