SPECIFIC GENETIC FEATURES OF TUBERCULOUS MYCOBACTERIA IN URAL FEDERAL DISTRICT OF RUSSIA

The article presents molecular genetic description of isolates of Mycobacterium tuberculosis of 178 new patients and 78 patients who had been previously treated obtained in 2009-2011 on the territory of Urals. PCR in real time and MIRU-VNTR typing of 15 loci detected prevalence of M. tuberculosis of Beijing genotype in the both groups of patients, however the part of Beijing isolates was significantly higher in the group of patients who had been previously treated compared to new patients: 80.8% versus 55.1% (p = 0.0002). IS6110-RFLP-typing showed that the majority of Beijing isolates belonged to B0 clone, clinically and epidemiologically significant in Russia. It was found out that M. tuberculosis Beijing carrying mutations rpoB Ser531→Leu and katG Ser315→Thr played the major role in MDR tuberculosis transmission in Urals. Non-Beijing group was represented by isolates of various spoligotypes and MIRU-VNTR types among which the representatives of the following globally common genetic groups prevailed: LAM (LAM9, T5_RUS), URAL (Н3), Haarlem (H1, X), Т (Т1).

Mycobacterium tuberculosis, MIRU-VNTR, IS6110-RFLP, Beijing, non-Beijing, Mycobacterium tuberculosis, genotyping, MIRU-VNTR, spoligotyping, IS6110-RFLP, Beijing, non-Beijing

1. Balabanova YA.M., Nikolaevskiy V.V., Radi M. et al. Prevalence of Mycobacterium tuberculosis strains of Beijing family and risk factors of their transmission in Samara Region. Probl. Tub., 2006, no. 2, pp. 31-36. (In Russ.)

2. Baranov А.А., Mariandyshev А.O. Correlations between genotypes and drug resistance of tuberculosis mycobacteria in the Barents Region of the Russian Federation. Ross. Med. Journal, 2009, no. 1, pp. 24-26. (In Russ.)

3. Vyazovaya А.А., Mokrousov I.V., Otten T.F. et al. Molecular and genetic characteristics of Mycobacterium tuberculosis strains circulating in Pskov Region. Tub. i Bolezni Legkikh, 2012, no. 6, pp. 35-39. (In Russ.)

4. Eremeeva N.I., Kravchenko M.А., Vakhrusheva D.V. et al. Evaluation of environmental contamination of TB hospital as a component of infection control system. Med. Alyans, 2013, no. 4, pp. 41-52. (In Russ.)

5. Eremeeva N.I., Kravchenko M.А., Kanishhev V.V. et al. Molecular genetic and phenotypic properties of hospital strains of tuberculous mycobacteria. Infektsiya i Immunitet, 2014, Special Issue, pp. 79. (In Russ.)

6. Lats А.А., Zhdanova S.N., Ogarkov O.B. et al. Drug resistance of various genotypes of Mycobacterium tuberculosis in tuberculosis patients in Irkutsk Region. Izvestiya Irkutskogo Gosudarstvennogo Universiteta, 2011, vol. 4, no. 4, pp. 58-62. (In Russ.)

7. Mokrousov I.V., Narvskaya O.V., Vyazovaya А.А. et al. Gene identification of epidemiologically and clinically significant variant of M. tuberculosis Beijing B0/W148. Tub. i Bolezni Legkikh, 2012, no. 10, pp. 33-36. (In Russ.)

8. Narvskaya O.V. Genomny polimorfizm Mycobacterium tuberculosis i ego rol v epidemicheskom protsesse. Diss. dokt. med. nauk. [Genome polymorphism of Mycobacterium tuberculosis and its role for epidemiological process. Doct. Diss.]. St. Petersburg, 2003.

9. Ogarkov O.B., Medvedeva T.V., Zozio Т. Molecular typing of tuberculosis mycobacteria strains in Irkutsk Region (Eastern Siberia) in 2000-2005. Molekulyarnaya Meditsina, 2007, no. 2, pp. 33-38. (In Russ.)

10. Edict no. 109 by RF MoH as of 21.03.2003 On Improvement of TB Control Measures in the Russian Federation. (In Russ.)

11. Bifani P.J. Plikaytis B.B., Kapur V. et al. Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family. JAMA, 1996, vol. 275, pp. 452-457.

12. Comas I., Homolka S., Niemann S. et al. Genotyping of genetically monomorphic bacteria: DNA sequencing in Mycobacterium tuberculosis highlights the limitations of current methodology. PLoS One, 2009, vol. 4, no. 11.

13. Demay C., Liens B., Burguiere Т. et al. SITVITWEB – A publicly available international multimarker database for studying Mycobacterium tuberculosis genetic diversity and molecular epidemiology. Infect.Genet. Evol., 2012, vol. 12, no. 4, pp. 755-766.

14. Dubiley S. Ignatova A., Mukhina T. et al. Molecular epidemiology of tuberculosis in Tula area, central Russia, before Directly Observed Therapy Strategy. Clin. Microbiol. Infect., 2010, vol. 16, no. 9, pp. 1421-1426.

15. van Embden J.D., Cave M.D., Crawford J.T. et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J. Clin. Microbiol., 1993, no. 31, pp. 406-409.

16. Iwamoto T. Yoshida S., Suzuki K. et al. Hypervariable loci that enhance the discriminatory ability of newly proposed 15-loci and 24-loci variable-number tandem repeat typing method on Mycobacterium tuberculosis strains predominated by the Beijing family. FEMS Microbiol. Lett., 2007, vol. 272, pp. 282-283.

17. Kamerbeek J., Schouls L., Kolk A. et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J. Clin. Microbiol., 1997, no. 35, pp. 907-914.

18. Kovalev S.Y., Kamaev E.Y., Kravchenko M.A. et al. Genetic analysis of Mycobacterium tuberculosis strains isolated in Ural region, Russian Federation, by MIRU-VNTR genotyping. International J. Tub. Lung Disease, 2005, no. 9, pp. 746-752.

19. Kremer K., Arnold C., Cataldi A. et al. Discriminatory power and reproducibility of novel DNA typing methods for Mycobacterium tuberculosis complex strains. J. Clin. Microbiol., 2005, no. 43, pp. 5628-5638.

20. Kubica T. Rüsch-Gerdes S., Niemann S. et al. The Beijing genotype is emerging among multidrug-resistant Mycobacterium tuberculosis strains from Germany. Int. J. Tuberc. Lung Dis., 2004, vol. 8, no. 9, pp. 1107-1113.

21. Kubica T., Agzamova R., Wright A. et al. The Beijing genotype is a major cause of drug-resistant tuberculosis in Kazakhstan. Int. J. Tuberc. Lung Dis., 2005, vol. 9, no. 6, pp. 646-653.

22. Kurepina N. Ramaswamy S., Shashkina E. et al. The sequence analysis of the pncA gene determining the PZA-resistance in the predominant M. tuberculosis strains isolated in the Tomsk penitentiary system, Western Siberia, Russia. Int. J. Tuberc. Lung Dis., 2001, vol. 5, no. 11, pp. 41.

23. Mokrousov I.O., Narvskaya A., Vyazovaya et al. Mycobacterium tuberculosis Beijing genotype in Russia: in search of informative VNTR loci. J. Clin. Microbiol., 2008, no. 46, pp. 3576-3584.

24. Supply P., Allix C., Lesjean S. et al. Proposal for Standardization of Optimized Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat Typing of Mycobacterium tuberculosis. J. Clin. Microbiol., 2006, no. 44, pp. 4498-4510.