TRANSPLANTATION OF AUTOLOGOUS MESENCHYMAL STEM CELLS IN THE EXPERIMENTAL MYCOBACTERIAL INFECTION: IMPACT OF CONDITIONING
Abstract
The use of mesenchymal stem cells (MSC) in immune therapy of drug resistant tuberculosis is to be experimentally justified. The impact of non-conditioned and poly(A:U)-conditioned MSC on the course of BCG-infection in mice has been studied. It has been proved that double administration of MSC to mice of BALB/c line in 11 and 12.5 weeks after infection with BCG caused significant reduction of granulomas formation and activated the mycobacterial growth. On the contrary the similar administration of MSC with poly(A:U) conditioning significantly inhibited the mycobacterial growth and provided no effect on granulomas formation. Non-conditioned MSC manifested immune suppression properties, and poly(A:U) conditioning caused their pro-inflammatory polarization in vitro. It has been concluded that MSC therapy of mycobacterial infection can be effective in case of targeted formation of pro-inflammatory immune-phenotype of MSC.
About the Authors
Ya. Sh. Shvarts
Research Institute of Therapy and Preventive Medicine
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
S. N. Belogorodtsev
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
P. N. Filimonov
Novosibirsk Tuberculosis Research Institute
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
A. G. Cherednichenko
Novosibirsk Tuberculosis Research Institute
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
References
1. Vasil’eva I.А., Konoplyannikov А.G., Erokhin V.V. et al. Curative effect of system transplantation of cultured autogenous mesenchymal stem cells of bone marrow in patients suffering from resistant forms of pulmonary tuberculosis. Kletochnaya Transplantologiya i Tkanevaya Inzheneriya, 2007, vol. 2, no. 1, pp. 77-80. (In Russ.)
2. Kalinina N.I., Sysoeva V.Yu., Rubina K.А. Mesenchymal stem cells in tissue growth and reparation. Acta Naturae (Russian version), 2011, vol. 3, no. 4(11), pp. 32-39. (In Russ.)
3. Skryagina E.M., Skryagin А.E., Isaykina Ya.I. et al. Treatment of drug resistant tuberculosis patients with the use of autologous transplantation of mesenchymal stem cells. Retsept, 2011, no. 2 (76), pp. 84-93. (In Russ.)
4. Das B., Kashino S.S., Pulu I. et al. CD271+ bone marrow mesenchymal stem cells may provide a niche for dormant Mycobacterium tuberculosis. Sci. Transl. Med., 2013, vol. 5, Issue 170. pp. ra13.
5. Dumitru C.A., Hemeda H., Jakob M. et al. Stimulation of mesenchymal stromal cells (MSCs) via TLR3 reveals a novel mechanism of autocrine priming. FASEB J., 2014, vol. 28, no. 9, pp. 3856-3866.
6. Hawn T.R., Matheson A.I., Maley S.N. et al. Host-Directed Therapeutics for Tuberculosis: Can We Harness the Host? Microbiol. Mol. Biol. Rev., 2013. vol. 77, pp. 608-627.
7. Modlin R.L., Bloom B.R. TB or Not TB: That Is No Longer the Question. Sci. Transl. Med., 2013, vol. 5, issue 213, pp. sr6.
8. Raghuvanshia S., Sharmaa P., Singhb S. et al. Mycobacterium tuberculosis evades host immunity by recruiting mesenchymal stem cells. PNAS, 2010, 107, no. 50, pp. 21653-21658.
9. Ren G., Chen X., Dong F. et al. Concise review: mesenchymal stem cells and translational medicine: emerging issues. Stem. Cells Transpl. Med., 2012, vol. 1, no. 1, pp. 51-58.
10. Romieu-Mourez R., Francois M., Boivin M.-N. et al. Cytokine Modulation of TLR Expression and Activation in Mesenchymal Stromal Cells Leads to a Proinflammatory Phenotype. J. Immunol., 2009, vol. 182, pp. 7963-7973.
11. Salem H.K., Thiemermann C. Mesenchymal stromal cells: current understanding and clinical status. Stem Cells, 2010, vol. 28, pp. 585-596.
12. Sendide K., Deghmane A.-E., Reyrat J.-M. et al. Mycobacterium bovis BCG urease attenuates major histocompatibility complex class II trafficking to the macrophage cell surface. Infect.Immun., 2004, vol. 72, no. 7, pp. 4200-4209.
13. Sun J., Deghmane A.-E., Soualhine H. et al. Mycobacterium bovis BCG disrupts the interaction of Rab7 with RILP contributing to inhibition of phagosome maturation. J. Leukoc. Biol., 2007, vol. 82, no. 6, pp. 1437-1445.
14. Tropel Ph., Noël D., Platet N. et al. Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Exp. Cell. Research, 2004, vol. 295, pp. 395-406.
15. Waterman R.S., Tomchuck S.L., Henkle S.L. et al. A new mesenchymal stem cell (MSC) paradigm: Polarization into a pro-inflammatory MSC1 or an immunosuppressive MSC2 phenotype. PLoS One, 2010, vol. 5, issue 4, pp. e10088.
Review
For citations:
Shvarts Ya.Sh., Belogorodtsev S.N., Filimonov P.N., Cherednichenko A.G. TRANSPLANTATION OF AUTOLOGOUS MESENCHYMAL STEM CELLS IN THE EXPERIMENTAL MYCOBACTERIAL INFECTION: IMPACT OF CONDITIONING. Tuberculosis and Lung Diseases. 2015;(12):31-36. (In Russ.)
Views:
618
Email this article 