INTERACTION OF T-LYMPHOCYTES OF CD4+CD27HI AND CD4+CD27LO WITH MACROPHAGES IN TUBERCULOUS INFECTION IN MICE
Abstract
Greatly the protective immune response in tuberculosis is defined by the ability of activated pulmonary macrophages of the host to suppress the mycobacterial growth. Macrophages are activated by the action of cytokines and other inflammatory mediators, in particular IFN-γ and TNF-α, secreted by T-lymphocytes. There are two different populations of effector lymphocytes of CD4+CD62LloCD44hi, one of them has the surface phenotype of CD27hi, and the other – CD27lo. Early the experimental model of tuberculous infection has shown that these types of cells have different vitality and abilities to produce inflammatory cytokines of IFN-γ and TNF-α. The effect of these cells on bacteriostatic activity of macrophages has been studied. It has been shown that Т-lymphocytes of CD27lo are much more effcient activating macrophages and stronger promote formation of active nitrogen forms. Activating action of the effectors of CD27lo was observed even when the ratio of macrophages and T-cells made 625 : 1. With this ratio the active nitrogen forms have not been detected thus one can conclude that there is one more nitrogen independent activation mechanism.
About the Authors
G. S. Syepel'kova
Central Tuberculosis Research Institute
Russian Federation
Moscow
Russian Federation
Moscow
K. B. Mayorov
Central Tuberculosis Research Institute
Russian Federation
Moscow
Russian Federation
Moscow
V. V. Evstifeev
Central Tuberculosis Research Institute
Russian Federation
Moscow
Russian Federation
Moscow
A. S. Apt
Central Tuberculosis Research Institute
Russian Federation
Moscow
Russian Federation
Moscow
References
1. Apt A.S., Avdienko V.G., Nikonenko B.V. et al. Distinct H-2 complex control of mortality, and immune responses to tuberculosis infection in virgin and BCG-vaccinated mice. Clin. Exp. Immunol., 1993, vol. 94, no. 2, pp. 322-329.
2. Arens R., Tesselaar K., Baars P.A. et al. Constitutive CD27/CD70 interaction induces expansion of effector-type T cells and results in IFNgamma-mediated B cell depletion. Immunity, 2001, vol. 15, no. 5, pp. 801-812.
3. Brown G.R., Meek K., Nishioka Y. et al. CD27-CD27 ligand/CD70 interactions enhance alloantigen-induced proliferation and cytolytic activity in CD8+ T lymphocytes. J. Immunol., 1995, vol. 154, no. 8, pp. 3686-3695.
4. Flynn J.L., Chan J., Lin P.L. Macrophages and control of granulomatous inflammation in tuberculosis. Mucosal. Immunol., 2011, vol. 4, no. 3, pp. 271-278.
5. Hoffman J.A., Kafatos F., Janeway Jr.C. et al. Phylogenetic perspectives in innate immunity. Science, 1999, vol. 284, pp. 1313-1318.
6. Kapina M.A., Shepelkova G.S., Mischenko V.V. et al. CD27low CD4 T lymphocytes that accumulate in the mouse lungs during mycobacterial infection differentiate from CD27high precursors in situ, produce IFN-gamma, and protect the host against tuberculosis infection. J. Immunol., 2007, vol. 178, no. 2, pp. 976-985.
7. Lyadova I.V., Oberdorf S., Kapina M.A. et al. CD4 T cells producing IFN-gamma in the lungs of mice challenged with mycobacteria express a CD27-negative phenotype. Clin. Exp. Immunol., 2004, vol. 138, no. 1, pp. 21-29.
8. MacMicking J.D., North R.J., LaCourse R. et al. Identification of nitric oxide synthase as a protective locus against tuberculosis. Proc. Natl. Acad. Sci. U.S.A., 1997, vol. 94, pp. 5243-5248.
9. Majorov K.B., Lyadova I.V., Kondratieva T.K. et al. Different innate ability of I/St and A/Sn mice to combat virulent Mycobacterium tuberculosis: phenotypes expressed in lung and extrapulmonary macrophages. Infect.Immun., 2003, vol. 71, no. 2, pp. 697-707.
10. Nikonenko B.V., Averbakh M.M., Lavebratt C. et al. Comparative analysis of mycobacterial infections in susceptible I/St and resistant A/Sn inbred mice. Tuber. Lung Dis., 2000, vol. 80, pp. 15-25.
11. Rowley T.F., Al-Shamkhani A. Stimulation by soluble CD70 promotes strong primary and secondary CD8+ cytotoxic T cell responses in vivo. J. Immunol., 2004, vol. 172, no. 10, pp. 6039-6046.
Review
For citations:
Syepel'kova G.S., Mayorov K.B., Evstifeev V.V., Apt A.S. INTERACTION OF T-LYMPHOCYTES OF CD4+CD27HI AND CD4+CD27LO WITH MACROPHAGES IN TUBERCULOUS INFECTION IN MICE. Tuberculosis and Lung Diseases. 2015;(12):57-60. (In Russ.)
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