MACROPHAGE AND CYTOKINE PROFILES OF BRONCHOALVEOLAR LAVAGE IN NEW AND RELAPSING RESPIRATORY SARCOIDOSIS
https://doi.org/10.21292/2075-1230-2016-94-9-59-64
Abstract
Goal of the study: to investigate structural and functional specific features of macrophages and cytokine profiles of bronchoalveolar lavage in new and relapsing respiratory sarcoidosis (RS).
Materials and methods. The study included comparative light optical and electronic microscopic examinations of macrophages, macrophage formula and cytokine profile of bronchoalveolar lavage in 120 patients suffering from different forms of RS. It was found that in case of new RS mostly macrophages of M1 phenotype were detected in the lavage. They had high level of IL-8, IL-2, IL-1β production and ultrastructural signs of hypersecretion which was especially typical of platycytes. High level of IL-4, IL-5 content is typical of relapsing sarcoidosis: additionally to macrophages of M1 phenotype, there are macrophages of M2 phenotype with ultrastructural signs of phagocytic function which can be used for diagnostics of this form of RS.
About the Authors
N. G. Demyanenko
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Junior Researcher of Department for Tuberculosis Differential Diagnostics and In Vitro Treatment Techniques,
2, Yauzskaya alleya, Moscow, 107564
L. N. Lepekha
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Doctor of Biological sciences, Professor, Head of Department for Pathoanatomy, Cellular Biology and Biochemistry,
2, Yauzskaya alleya, Moscow, 107564
E. I. Shmelev
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Doctor of Medical sciences, Professor, Head of Department for Tuberculosis Differential Diagnostics and In Vitro Treatment Techniques,
2, Yauzskaya alleya, Moscow, 107564
M. M. Averbakh
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Doctor of Medical sciences, senior Researcher of Immunology Department,
2, Yauzskaya alleya, Moscow, 107564
T. A. Statsuk
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Candidate of Medical sciences, Doctor of Clinical laboratory Diagnostics of Clinical Diagnostic Department,
2, Yauzskaya alleya, Moscow, 107564
I. V. Sivokozov
Central Tuberculosis Research Institute
Russian Federation
Russian Federation
Researcher of Clinical Diagnostic Department,
2, Yauzskaya alleya, Moscow, 107564
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Review
For citations:
Demyanenko N.G., Lepekha L.N., Shmelev E.I., Averbakh M.M., Statsuk T.A., Sivokozov I.V. MACROPHAGE AND CYTOKINE PROFILES OF BRONCHOALVEOLAR LAVAGE IN NEW AND RELAPSING RESPIRATORY SARCOIDOSIS. Tuberculosis and Lung Diseases. 2016;94(9):59-64. (In Russ.) https://doi.org/10.21292/2075-1230-2016-94-9-59-64
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