EVALUATION OF SPECIFIC ANTIGENS AT THE EARLY STAGE OF TUBERCULOUS INFECTION IN CHILDREN
https://doi.org/10.21292/2075-1230-2017-95-1-27-33
Abstract
More specific antigens are still being searched for in order to increase informativeness of techniques for early diagnostics of tuberculous infection. Aimed at the investigation of proteins of tuberculous mycobacteria (PPD-L, ESAT 6, 85, hybrid of ЕSAT 6 CFP 10, CFP32B, Rv2660c) the study was conducted including testing of induced IFN-γ in the children ill with tuberculosis in the early period of primary tuberculous infection and also those not infected with tuberculous mycobacteria – 130 children in the age from 6.0 ± 0.4 years old. It was found out that tuberculin remained to be valuable for evaluation of the intensity of anti-tuberculosis immunity. Antigens of ESAT 6, 85, CFP32B, Rv2660c were identified as proteins of the early stage of tuberculous infection. The presence of response when stimulating by the above antigens was typical of the favorable course of tuberculosis. Antigens of ESAT 6 and Rv2660c were valuable for assessment of latent tuberculous infections and the hybrid of ЕSAT 6 CFP 10 – for diagnostics of tuberculosis. Therefore introduction of immunological tests (in vitro) into the set of diagnostic tools allows optimizing the early diagnostics of tuberculous infection in children and forecasting the course of the disease when tuberculosis has developed.
About the Authors
M. A. Plekhanova
Omsk State Medical University
Russian Federation
Russian Federation
Associate Professor of Pediatric Department with Professional Development Training,
12, Lenina St., Omsk, 644043
V. A. Аksenova
Research Institute of Phthisiopulmonology by I. M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
Head of Department for Tuberculosis in Children and Adolescents, Chief External Children Phthisiologist,
8, Bd. 2, Trubetskaya St., Moscow, 119991
A. P. Tkachuk
Gamaleya Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Leading Researcher, Head of Laboratory of Translational Biomedicine by Department of Genetics and Molecular Biology of Bacteria,
18, Gamaleya St., Moscow, 123098
info@gamaleya.org
Yu. I. Patsula
Siberian Federal Regional Center on AIDS Prevention and Control, Omsk Research Institute of Natural Focal Infections
Russian Federation
Russian Federation
Senior Researcher of Arbitral Laboratory for Diagnostics of HIV and Opportunistic Infections,
7, Mira Ave., Omsk, 644080
L. A. Krivtsova
Omsk State Medical University
Russian Federation
Russian Federation
Head of Pediatric Department with Professional Development Training,
12, Lenina St., Omsk, 644043
A. N. Kolomeets
Siberian Federal Regional Center on AIDS Prevention and Control, Omsk Research Institute of Natural Focal Infections
Russian Federation
Russian Federation
Bacteriologist of Arbitral Laboratory for Diagnostics of HIV and Opportunistic Infections, Head of Molecular Diagnostics Laboratory of the Department for Natural Focal Bacterial Zoonoses,
7, Mira Ave., Omsk, 644080
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Review
For citations:
Plekhanova M.A., Аksenova V.A., Tkachuk A.P., Patsula Yu.I., Krivtsova L.A., Kolomeets A.N. EVALUATION OF SPECIFIC ANTIGENS AT THE EARLY STAGE OF TUBERCULOUS INFECTION IN CHILDREN. Tuberculosis and Lung Diseases. 2017;95(1):27-33. (In Russ.) https://doi.org/10.21292/2075-1230-2017-95-1-27-33
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